| Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. | |
| | |
MedLine Citation:
|
PMID: 20688228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 degrees C and 37 degrees C, reaching a maximum production of 1.0 x 10(9) AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. |
| | |
Authors:
|
Svetoslav Dimitrov Todorov; Monica Wachsman; Elisabetta Tomé; Xavier Dousset; Maria Teresa Destro; Leon Milner Theodore Dicks; Bernadette Dora Gombossy de Melo Franco; Manuella Vaz-Velho; Djamel Drider |
Related Documents
:
|
12501428 - Lactic acid bacteria found in fermented fish in thailand. 17186668 - Nonstarter lactobacilli isolated from soft and semihard argentinean cheeses: genetic ch... 9812378 - Use of bacteriocinogenic lactic acid bacteria to inhibit spontaneous nisin-resistant mu... 18481218 - Characterization of lactococcus lactis subsp. lactis isolated from surface waters. 11830938 - Plasmids of selenomonas ruminantium and development of host-vector system. 667408 - Salmonella infection in market swine, trinidad and tobago. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-31 |
Journal Detail:
|
Title: Food microbiology Volume: 27 ISSN: 1095-9998 ISO Abbreviation: Food Microbiol. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-08-06 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8601127 Medline TA: Food Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 869-79 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo-SP, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anti-Bacterial Agents / pharmacology Antibiosis Antiviral Agents / pharmacology Bacteriocins / biosynthesis, pharmacology* Colony Count, Microbial Consumer Product Safety Enterococcus faecium / metabolism, physiology* Food Microbiology Herpesvirus 1, Human / drug effects*, growth & development Humans Listeria / drug effects Listeria monocytogenes / drug effects*, growth & development Microbial Sensitivity Tests Molecular Weight Salmon / microbiology* Seafood / microbiology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Antiviral Agents; 0/Bacteriocins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fate of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches und...
Next Document: The effect of ripening and storage conditions on the distribution of tyramine, putrescine and cadave...