Document Detail

Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum.
MedLine Citation:
PMID:  25111749     Owner:  NLM     Status:  Publisher    
This study systematically investigated the different types of LH2 produced by Allochromatium (Alc.) vinosum, a photosynthetic purple sulphur bacterium, in response to variations in growth conditions. Three different spectral forms of LH2 were isolated and purified, the B800-820, B800-840 and B800-850 LH2 types, all of which exhibit an unusual split 800 peak in their low temperature absorption spectra. However, it is likely that more forms are also present. Relatively more B800-820 and B800-840 are produced under low light conditions, while relatively more B800-850 is produced under high light conditions. Polypeptide compositions of the three different LH2 types were determined by a combination of HPLC and TOF/MS. The B800-820, B800-840 and B800-850 LH2 types all have a heterogeneous polypeptide composition , containing multiple types of both α and β polypeptides, and differ in their precise polypeptide composition. They all have a mixed carotenoid composition, containing carotenoids of the spirilloxanthin series. In all cases the most abundant carotenoid is rhodopin; however there is a shift towards carotenoids with a higher conjugation number in LH2 complexes produced under low light conditions. CD spectroscopy, together with the polypeptide analysis, demonstrates that these Alc. vinosum LH2 complexes are more closely related to the LH2 complex from Phs. molischianum than they are to the LH2 complexes from Rps. acidophila.
Anne-Marie Carey; Kirsty Hacking; Nichola Picken; Suvi Honkanen; Sharon Kelly; Dariusz M Niedzwiedzki; Robert E Blankenship; Yuuki Shimizu; Zheng-Yu Wang-Otomo; Richard J Cogdell
Related Documents :
20072269 - Detector response and perfect-lens-mtf in polychromatic light.
961819 - Diffraction theory and visual hyperacuity.
20072209 - Absolute calibration of light sources in the vacuum ultraviolet by means of the synchro...
25405839 - Effects of fast, slow, and adaptive amplitude compression on children's and adults' per...
18345189 - Improved design for stray-light reduction with a hemispherical imager.
23226369 - Optogenetic control of transcription in zebrafish.
23312729 - Effects of moving forward or backward on the saddle on knee joint forces during cycling.
22068599 - Modulation of rod photoreceptor output by hcn1 channels is essential for regular mesopi...
9405529 - Conditioning-related protection from acoustic injury: effects of chronic deefferentatio...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-8
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  -     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier B.V.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  BBA-Bioenergetics special issue on "Vibrational Spectroscopies and Bioenergetic Systems" Infrared sp...
Next Document:  Connection between the membrane electron transport system and Hyn hydrogenase in the purple sulfur b...