Document Detail


Characterisation of EstCOo8 and EstC34, intracellular esterases, from the wine associated lactic acid bacterium Oenococcus oeni and Lactobacillus hilgardii.
MedLine Citation:
PMID:  23134121     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To clone and characterise two related intracellular esterases from Oenococcus oeni and Lactobacillus hilgardii under wine-like conditions. METHODS AND RESULTS: The published genome sequences for O. oeni and Lb. hilgardii were used to identify, clone and purify putative esterase genes from these species designated EstCOo8 and EstC34 respectively. Both esterases are members of family V of lipolytic enzymes. However EstC34 contains an SGSLG nucleophilic elbow structural motif instead of the usual GGSLG motif which is conserved in other lactic acid bacteria. Both esterases exhibited greatest specificity for C(2) to C(4) pNP-linked substrates and retained activity under wine-like conditions. EstCOo8 had an optimum temperature, pH, and ethanol concentration of 40°C, 5.5 and 6% (vol/vol), respectively. Whereas EstC34 had an optimum temperature, pH and ethanol concentration of 50°C, 5.0 and 10% (vol/vol), respectively. CONCLUSIONS: Both esterases were stable and retained activity under conditions that would be encountered in wine. They have the potential to reduce short chain ethyl esters such as ethyl acetate. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides information that might help improve the performance of LAB during malolactic fermentation in wine in the future, either by strain selection, optimisation or direct enzyme addition. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Krista M Sumby; Paul R Grbin; Vladimir Jiranek
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
School of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064.
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