| Characterisation of the ArmA adenylation domain implies a more diverse secondary metabolism in the genus Armillaria. | |
| | |
MedLine Citation:
|
PMID: 21802058 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The armA-gene, encoding a tridomain enzyme reminiscent of nonribosomal peptide synthetases, was identified in the genome of the basidiomycete Armillaria mellea. Heterologously expressed enzyme and the ATP-pyrophosphate exchange assay were used for the in vitro biochemical characterisation of the ArmA adenylation domain. l-leucine was the preferred substrate, while l-threonine, l-valine, l-alanine, and l-isoleucine were turned over at lower rates (83 %, 62 %, 56 %, and 44 %, respectively). Other proteinogenic amino acids, 2-oxo acids, and benzoic acid derivatives were not accepted. As the substrate range of ArmA is incompatible with the secondary metabolites known from the genus Armillaria, our results imply greater natural product diversity in this genus. This is the first biochemical characterisation of a basidiomycete amino acid-adenylating domain, and our results may help refine computer algorithms to predict substrate specificities for basidiomycete nonribosomal peptide synthetases whose genes are discovered through genome sequencing. |
| | |
Authors:
|
Mathias Misiek; Jana Braesel; Dirk Hoffmeister |
Related Documents
:
|
20565608 - The uromyces fabae ufaat3 gene encodes a general amino acid permease that prefers uptak... 16466078 - Gender differences in amino acid use during endurance exercise. 11541888 - Delivery of extraterrestrial amino acids to the primitive earth. exposure experiments i... 22247238 - Safety assessment of xylene sulfonic acid, toluene sulfonic acid, and alkyl aryl sulfon... 22312728 - Ferric reducing, antiradical and beta-carotene bleaching activities of nicotinic acid a... 2006908 - Purification and characterization of acidic glutathione s-transferase 6 from human brain. |
Publication Detail:
|
Type: Journal Article Date: 2011-06-30 |
Journal Detail:
|
Title: Fungal biology Volume: 115 ISSN: 1878-6146 ISO Abbreviation: Fungal Biol Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-08-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101524465 Medline TA: Fungal Biol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 775-81 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
|
Friedrich-Schiller-Universität Jena, Department Pharmaceutical Biology at the Hans-Knöll-Institute, Beutenbergstrasse 11a, 07745 Jena, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: No evidence for a Ganoderma spore dispersal mutualism in an obligate spore-feeding beetle Zearagytod...
Next Document: Lipid content disturbance in the arbuscular mycorrhizal, Glomus irregulare grown in monoxenic condit...