Document Detail

Characterisation of the ArmA adenylation domain implies a more diverse secondary metabolism in the genus Armillaria.
MedLine Citation:
PMID:  21802058     Owner:  NLM     Status:  In-Data-Review    
The armA-gene, encoding a tridomain enzyme reminiscent of nonribosomal peptide synthetases, was identified in the genome of the basidiomycete Armillaria mellea. Heterologously expressed enzyme and the ATP-pyrophosphate exchange assay were used for the in vitro biochemical characterisation of the ArmA adenylation domain. l-leucine was the preferred substrate, while l-threonine, l-valine, l-alanine, and l-isoleucine were turned over at lower rates (83 %, 62 %, 56 %, and 44 %, respectively). Other proteinogenic amino acids, 2-oxo acids, and benzoic acid derivatives were not accepted. As the substrate range of ArmA is incompatible with the secondary metabolites known from the genus Armillaria, our results imply greater natural product diversity in this genus. This is the first biochemical characterisation of a basidiomycete amino acid-adenylating domain, and our results may help refine computer algorithms to predict substrate specificities for basidiomycete nonribosomal peptide synthetases whose genes are discovered through genome sequencing.
Mathias Misiek; Jana Braesel; Dirk Hoffmeister
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Publication Detail:
Type:  Journal Article     Date:  2011-06-30
Journal Detail:
Title:  Fungal biology     Volume:  115     ISSN:  1878-6146     ISO Abbreviation:  Fungal Biol     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524465     Medline TA:  Fungal Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  775-81     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Friedrich-Schiller-Universität Jena, Department Pharmaceutical Biology at the Hans-Knöll-Institute, Beutenbergstrasse 11a, 07745 Jena, Germany.
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