| Chapter 5. Avian embryos a model for the study of primary vascular assembly in warm-blooded animals. | |
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MedLine Citation:
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PMID: 19022057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The formation of a primary vascular bed is a dynamic process, aspects of which are readily amenable to time-lapse imaging in avian embryos. At early developmental stages, the body plan of avian embryos is very similar to mammals and has many properties that make it ideal for imaging. We devised labeling, culturing, and imaging techniques that capture high-resolution images of intact avian embryos in four dimensions over large length scales (1 to 5000 microm). Here, we describe multiple techniques for labeling and culturing avian embryos to study the cellular, tissue, and extracellular matrix dynamics of vascular morphogenesis. |
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Authors:
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Paul A Rupp; Mike B Filla; Cheng Cui; Charles D Little |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 445 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-04-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 107-23 Citation Subset: IM |
Affiliation:
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Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Birds / embryology* Blood Vessels / enzymology* Embryo, Nonmammalian / blood supply Quail / embryology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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