| Chaperone-like activity and surface hydrophobicity of 70S ribosome. | |
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MedLine Citation:
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PMID: 12220666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ribosomes have been shown to mediate refolding of proteins in vitro. In order to understand the mechanism of action, we have explored the 70S ribosome surface for hydrophobicity, one of the important aspects in chaperone-target protein interaction. We find that the 70S ribosome displays significant hydrophobicity on its surface when probed with the hydrophobic fluorophore 8-anilino-1-naphthalene sulfonate. To understand the functional significance of this hydrophobicity we investigated the ability of the ribosome to prevent aggregation of insulin B chain and alpha-lactalbumin induced by reducing the interchain and intrachain disulfide bond respectively with dithiothreitol (DTT) and photo aggregation of gamma-crystallin at 37 degrees C. The 70S ribosome offers complete protection towards light-induced aggregation of gamma-crystallin (at 1:2 (w/w) ratio of crystallin:ribosome) and DTT-induced aggregation of alpha-lactalbumin (at 1:3) and there is appreciable protection (at 1:3) against the aggregation of insulin B chain. We also investigated the role of 70S ribosome in refolding of bovine carbonic anhydrase. Ribosomes improved the folding yield in a concentration-dependent manner. These results clearly demonstrate a general chaperone-like activity of 70S ribosome and implicate its surface hydrophobicity. |
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Authors:
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Ranvir Singh; Ch Mohan Rao |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 527 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-09-10 Completed Date: 2002-11-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 234-8 Citation Subset: IM |
Affiliation:
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Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anilino Naphthalenesulfonates
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chemistry Carbonic Anhydrases / chemistry Disulfides / chemistry Dithiothreitol / chemistry Hydrophobicity Insulin / chemistry Lactalbumin / chemistry Molecular Chaperones / metabolism* Ribosomes / chemistry*, metabolism* gamma-Crystallins / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Anilino Naphthalenesulfonates; 0/Disulfides; 0/Molecular Chaperones; 0/gamma-Crystallins; 11061-68-0/Insulin; 3483-12-3/Dithiothreitol; 82-76-8/1-anilino-8-naphthalenesulfonate; 9013-90-5/Lactalbumin; EC 4.2.1.1/Carbonic Anhydrases |
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