| Channelrhodopsin as a tool to investigate synaptic transmission and plasticity. | |
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MedLine Citation:
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PMID: 20562296 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The light-gated cation channel channelrhodopsin-2 (ChR2) has been used in a variety of model systems to investigate the function of complex neuronal networks by stimulation of genetically targeted neurons. In slice physiology, ChR2 opens the door to novel types of experiments and greatly extends the technical possibilities offered by traditional electrophysiology. In this short review, we first consider several technical aspects concerning the use of ChR2 in slice physiology, providing examples from our own work. More specifically, we discuss differences between light-evoked action potentials and spontaneous or electrically induced action potentials. Our work implies that light-evoked action potentials are associated with increased calcium influx and a very high probability of neurotransmitter release. Furthermore, we point out the factors limiting the spatial resolution of ChR2 activation. Secondly, we discuss how synaptic transmission and plasticity can be studied using ChR2. Postsynaptic depolarization induced by ChR2 can be combined with two-photon glutamate uncaging to potentiate visually identified dendritic spines. ChR2-mediated stimulation of presynaptic axons induces neurotransmitter release and reliably activates postsynaptic spines. In conclusion, ChR2 is a powerful tool to investigate activity-dependent changes in structure and function of synapses. |
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Authors:
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Philipp Schoenenberger; Yan-Ping Zhang Schärer; Thomas G Oertner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-18 |
Journal Detail:
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Title: Experimental physiology Volume: 96 ISSN: 1469-445X ISO Abbreviation: Exp. Physiol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002940 Medline TA: Exp Physiol Country: England |
Other Details:
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Languages: eng Pagination: 34-9 Citation Subset: IM |
Affiliation:
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Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. philipp.schoenenberger@fmi.ch |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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