| Changing activities of galactose-metabolizing enzymes during perfusion of suckling-rat liver. | |
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MedLine Citation:
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PMID: 6259949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The specific activities of the galactose-metabolizing enzymes, galactokinase (EC 2.7.1.6), galactose-1-phosphate uridyltransferase (EC 2.7.7.12), and UDPgalactose 4-epimerase (EC 5.1.3.2), in suckling-rat livers perfused for 90 min with 1 and 4 mM galactose fluctuate significantly with a different pattern of change for each enzyme. Perfusion for 30 min with galactose resulted in a significant increase of transferase specific activity followed by a precipitous decline to about one-fifth of the activity in unperfused liver at 90 min. The increase in transferase activity was also observed when D-glucose was perfused but not when L-glucose, D-fructose, D-xylose, or D-ribose was added to the perfusate. No such changes in transferase activity were observed when adult-rat liver was perfused with galactose. Epimerase activity in the suckling-rat liver was relatively low, and the changes in its activity correlated best with the uptake rate of galactose. The perfused suckling-rat liver may provide a model system for examination of factors that modulate the specific activity of galactose-metabolizing enzymes and effect the metabolism of galactose. |
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Authors:
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S Rogers; S Segal |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 240 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1981 Mar |
Date Detail:
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Created Date: 1981-05-26 Completed Date: 1981-05-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E333-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Suckling Carbohydrate Epimerases / metabolism* Female Galactokinase / metabolism* Galactose / administration & dosage, metabolism* Liver / enzymology*, metabolism Male Nucleotidyltransferases / metabolism* Perfusion Rats Time Factors UDPglucose 4-Epimerase / metabolism* UTP-Hexose-1-Phosphate Uridylyltransferase / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HD-08536/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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26566-61-0/Galactose; EC 2.7.1.6/Galactokinase; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.10/UTP-Hexose-1-Phosphate Uridylyltransferase; EC 5.1.3.-/Carbohydrate Epimerases; EC 5.1.3.2/UDPglucose 4-Epimerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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