Document Detail

Changing Mad2 levels affects chromosome segregation and spindle assembly checkpoint control in female mouse meiosis I.
MedLine Citation:
PMID:  18043727     Owner:  NLM     Status:  MEDLINE    
The spindle assembly checkpoint (SAC) ensures correct separation of sister chromatids in somatic cells and provokes a cell cycle arrest in metaphase if one chromatid is not correctly attached to the bipolar spindle. Prolonged metaphase arrest due to overexpression of Mad2 has been shown to be deleterious to the ensuing anaphase, leading to the generation of aneuploidies and tumorigenesis. Additionally, some SAC components are essential for correct timing of prometaphase. In meiosis, we and others have shown previously that the Mad2-dependent SAC is functional during the first meiotic division in mouse oocytes. Expression of a dominant-negative form of Mad2 interferes with the SAC in metaphase I, and a knock-down approach using RNA interference accelerates anaphase onset in meiosis I. To prove unambigiously the importance of SAC control for mammalian female meiosis I we analyzed oocyte maturation in Mad2 heterozygote mice, and in oocytes overexpressing a GFP-tagged version of Mad2. In this study we show for the first time that loss of one Mad2 allele, as well as overexpression of Mad2 lead to chromosome missegregation events in meiosis I, and therefore the generation of aneuploid metaphase II oocytes. Furthermore, SAC control is impaired in mad2+/- oocytes, also leading to the generation of aneuploidies in meiosis I.
Théodora Niault; Khaled Hached; Rocío Sotillo; Peter K Sorger; Bernard Maro; Robert Benezra; Katja Wassmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-28
Journal Detail:
Title:  PloS one     Volume:  2     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-28     Completed Date:  2008-08-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1165     Citation Subset:  IM    
CNRS UMR7622 Biologie du Développement, Paris, France.
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MeSH Terms
Cell Cycle Proteins / physiology*
Mitotic Spindle Apparatus*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/MAD2 protein, mouse

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