| Changes to the rumen bacterial population of sheep with the addition of 2,4,6-trinitrotoluene to their diet. | |
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MedLine Citation:
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PMID: 20607404 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Previous work has shown that bacterial isolates from the sheep rumen are capable of detoxifying 2,4,6-trinitrotoluene (TNT) into polar constituents. In this study, the dietary effects of TNT on the sheep rumen microbial community were evaluated using molecular microbiology ecology tools. Rumen samples were collected from sheep fed with and without TNT added to their diet, genomic DNA was extracted, and the 16S rRNA-V3 gene marker was used to quantify changes in the microbial population in the rumen. Control and treatment samples yielded 533 sequences. Phylogenetic analyses were performed to determine the microbial changes between the two conditions. Results indicated the predominant bacterial populations present in the rumen were comprised of the phyla Firmicutes and Bacteroidetes, irrespective of presence/absence of TNT in the diet. Significant differences (P < 0.001) were found between the community structure of the bacteria under TNT (-) and TNT (+) diets. Examination of the TNT (+) diet showed an increase in the clones belonging to family Ruminococcaceae, which have previously been shown to degrade TNT in pure culture experiments. |
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Authors:
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Sudeep Perumbakkam; Edward A Mitchell; A Morrie Craig |
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Publication Detail:
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Type: Journal Article Date: 2010-07-06 |
Journal Detail:
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Title: Antonie van Leeuwenhoek Volume: 99 ISSN: 1572-9699 ISO Abbreviation: Antonie Van Leeuwenhoek Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372625 Medline TA: Antonie Van Leeuwenhoek Country: Netherlands |
Other Details:
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Languages: eng Pagination: 231-40 Citation Subset: IM |
Affiliation:
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Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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