Document Detail


Changes of proliferation kinetics after X-irradiation of a human malignant melanoma grown in nude mice.
MedLine Citation:
PMID:  6540185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human malignant melanoma grown in nude mice was exposed to single-dose X-irradiation and the effect on the proliferation kinetics was investigated by two methods. Flow cytometric DNA analysis was performed on tumour tissue obtained by sequential fine-needle aspirations after the treatment to monitor the initial cell cycle distribution changes. The technique of labelled mitoses was used to examine the kinetics of the tumours during regrowth. The results showed that the treatment initially induced a partial synchronization of small fractions of cells accumulated in the G2 phase of the cell cycle and a dose-dependent decrease of the cell generation time due to a shortening of the G1 duration time during regrowth of the tumours. Furthermore, it was shown that the calculated values of growth fraction and cell loss factor became unreliable because the tumours contained a dose-related increasing proportion of radiation-inactivated tumour cells.
Authors:
M Spang-Thomsen; L L Vindeløv
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  20     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-08-27     Completed Date:  1984-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  849-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / radiation effects
DNA, Neoplasm / metabolism
Dose-Response Relationship, Radiation
Flow Cytometry
Humans
Kinetics
Melanoma / metabolism,  pathology,  radiotherapy*
Mice
Mice, Nude
Mitosis / radiation effects
Neoplasm Transplantation
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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