Document Detail

Changes of ornithine decarboxylase activity and polyamine content upon differentiation of mouse NB-15 neuroblastoma cells.
MedLine Citation:
PMID:  6282899     Owner:  NLM     Status:  MEDLINE    
The possible functions of ornithine decarboxylase (ODC) and polyamines in the differentiation of mouse NB-15 neuroblastoma cells were investigated by examining the changes of these parameters in the differentiating and nondifferentiating NB-15 cells over a 5-day culture period. Differentiation of NB-15 cells was induced by the addition of dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine (1BMX) to the growth medium and was monitored by neurite outgrowth, increases of acetylcholinesterase (AChE), and RI cAMP-binding protein. Plating of NB-15 cells in fresh serum-containing growth medium was accompanied by rapid growth and a marked increase of ODC activity; this early increase of ODC activity was attenuated, both in duration and in magnitude, in the differentiating cells. The spermidine content of the differentiating neuroblastoma cells was significantly lower than that of the nondifferentiating cells. In the fully differentiated neuroblastoma cells, the ODC activity and spermidine content were lower than that of the undifferentiated cells by approximately 15-fold and five-fold, respectively. Based on these results it is proposed that changes of polyamine metabolism may be of significance in the differentiation of mouse neuroblastoma cells.
K Y Chen; V Presepe; N Parken; A Y Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  110     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-08-07     Completed Date:  1982-08-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  285-90     Citation Subset:  IM    
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MeSH Terms
Acetylcholinesterase / metabolism
Carboxy-Lyases / metabolism*
Cell Differentiation
Cell Line
Cyclic AMP / metabolism
Neurons / cytology*,  enzymology
Ornithine Decarboxylase / metabolism*
Polyamines / metabolism*
Putrescine / metabolism
Spermidine / metabolism
Spermine / metabolism
Grant Support
Reg. No./Substance:
0/Polyamines; 110-60-1/Putrescine; 124-20-9/Spermidine; 60-92-4/Cyclic AMP; 71-44-3/Spermine; EC; EC 4.1.1.-/Carboxy-Lyases; EC Decarboxylase

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