Document Detail

Changes of nucleotide content in human and rat heart during cardiac surgery and ischemia.
MedLine Citation:
PMID:  8140827     Owner:  NLM     Status:  MEDLINE    
The influence of ischemia on purine nucleotide and their catabolite concentration in human myocardium was investigated during surgery of acquired and congenital heart defects. This was compared with the influence of ischemia on rat heart. Concentrations of adenine and guanine nucleotides and their catabolites were measured in the extracts of heart biopsies taken at the onset of ischemia and at the time of reperfusion. The content of myocardial ATP in human heart decreased from the initial value of 22.3 +/- 1.1 to 14.6 +/- 1.5 nmol/mg protein and total adenine nucleotide pool decreased from 34.2 +/- 1.8 to 27.6 +/- 1.5 nmol/mg protein during the operation. Significant increases in myocardial concentrations of purine catabolites were also observed with the most prominent rise in inosine from below 0.5 at the onset of the ischemia to 3.0 +/- 0.5 nmol/mg protein at the time of reperfusion. A positive correlation was demonstrated between the concentration of purine catabolites in the heart at the end of ischemia with the decrease of both ATP and the total nucleotide pool. An interesting metabolic specificity of the ischemic human heart appeared to be only a small accumulation of inosine monophosphate (IMP). The increase of IMP in the rat heart after ischemia was several-fold higher. Thus, cardiac surgery of congenital and acquired heart defects was associated with a significant decrease in myocardial adenylate pool and a single biopsy collected at the end of ischemia seems to be sufficient to evaluate the extent of this metabolic and possibly functional impairment of the heart.
R T Smoleński; A C Składanowski; J Swierczyński; M Perko; M Narkiewicz; M M Zydowo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  40     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  1993  
Date Detail:
Created Date:  1994-04-25     Completed Date:  1994-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  531-8     Citation Subset:  IM    
Department of Biochemistry, Academic Medical School of Gdańsk, Poland.
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MeSH Terms
Adenine Nucleotides / metabolism
Energy Metabolism
Heart Arrest, Induced
Heart Defects, Congenital / metabolism,  surgery
Heart Diseases / metabolism,  surgery
Myocardial Ischemia / metabolism
Myocardium / metabolism*
Purine Nucleotides / metabolism*
Rats, Wistar
Species Specificity
Reg. No./Substance:
0/Adenine Nucleotides; 0/Purine Nucleotides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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