Document Detail


Changes of multiple biotransformation phase I and phase II enzyme activities in human fetal adrenals during fetal development.
MedLine Citation:
PMID:  18215353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: Fetal adrenal, which synthesizes steroid hormones, is critical to fetal growth and development. Our recent research showed that some xenobiotics could interfere with steroidogenesis and induce intrauterine growth retardation in rats. The study on the characteristics of biotransformation enzymes in fetal adrenals still seems to be important with respect to possible significance in xenobiotic-induced fetal development toxicity. In this study, the activities of several important xenobiotic-related phase I and phase II enzymes in human fetal adrenals were examined and compared with those in fetal livers. METHODS: The activity and mRNA expression were determined by enzymatic analysis and RT-PCR. RESULTS: The levels of cytochrome (CYP)2A6, CYP2E1, and CYP3A7 isozymes in fetal adrenals were 82%, 92%, and 33% of those in fetal livers, respectively. There was a good positive correlation between adrenal CYP2A6 activity and gestational time. The values of alpha glutathione S-transferase (GST), pi-GST, and microGST in adrenals were 0.5, 4.4, and 8.3-fold of those in the livers, respectively, and the activity of adrenal pi-GST was negatively correlated with gestational time. The uridine diphosphoglucuronyl transferase activities, which were measured using p-hydroxy-biphenyl and 7-hydroxy-4-methylcoumarin as substrates, were 9% and 3%, respectively, of those in the fetal livers. CONCLUSION: Our investigation suggested that adrenal could be an important xenobiotic-metabolizing organ in fetal development and may play a potential role in xenobiotic-induced fetal development toxicity.
Authors:
Hui Wang; Jie Ping; Ren-xiu Peng; Jiang Yue; Xue-yan Xia; Qi-xiong Li; Rui Kong; Jun-yan Hong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  29     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-24     Completed Date:  2009-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  231-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China. clbwhcbd@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / embryology*,  enzymology*
Adult
Cytochrome P-450 Enzyme System / metabolism
Female
Fetal Development / physiology
Gestational Age
Glutathione Transferase / metabolism
Humans
Liver / enzymology
Male
Pregnancy
Subcellular Fractions / enzymology
Xenobiotics / metabolism
Chemical
Reg. No./Substance:
0/Xenobiotics; 9035-51-2/Cytochrome P-450 Enzyme System; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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