Document Detail

Changes in tropomyosin during primary culture of embryonic myocardiocytes.
MedLine Citation:
PMID:  1628318     Owner:  NLM     Status:  MEDLINE    
We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompanied by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic. When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction. In the IFI analysis, tropomyosin was found to label the Stress fiber-like structures (SFL) in different patterns depending on the area of the cell which expressed this protein.
M A Muros; A E Aránega; C Vélez; F J González; J E Fernández; L Alvarez; A Aránega
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international reports     Volume:  16     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-08-19     Completed Date:  1992-08-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  451-64     Citation Subset:  IM    
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
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MeSH Terms
Cells, Cultured
Chick Embryo
Cytoplasm / metabolism
Cytoskeleton / metabolism
Fetal Heart / cytology*,  metabolism
Fluorescent Antibody Technique
Muscle Proteins / metabolism*
Tropomyosin / metabolism*
Reg. No./Substance:
0/Muscle Proteins; 0/Tropomyosin

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