Document Detail


Changes in transcriptional output of human peripheral blood mononuclear cells following resistance exercise.
MedLine Citation:
PMID:  21437602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various types of exercise alter the population of circulating peripheral blood mononuclear cells (PBMCs) and change their transcriptional output. This work examines changes in PBMC populations and transcription in response to resistance exercise training (RET), and identify key transcriptional changes in PBMCs that may play a role in altering peripheral tissues in response to RET. Ten resistance-trained men (20-24 years), performed an acute bout of RET for ~30 min following a 12 h fast. Venous blood was sampled at rest, immediately following exercise, and at 2 h post-exercise and analyzed for total and differential leukocytes and global gene expression using Affymetrix Genechips. Results showed elevated leukocytes, monocytes, lymphocytes, and lactate values immediately post-exercise (P < 0.05) over baseline. At 2 h post-exercise, leukocytes, and granulocytes remained elevated (P < 0.05), whereas lymphocytes were lower than (P < 0.05) baseline values. Initial microarray results showed the greatest transcriptional changes in pathways related to immune response, inflammation, and cellular communication. The change in PBMC population (2 h time point) correlated with a dramatic decrease in the expression of CD160, and XCL1, markers of lymphocyte populations. At the 2 h recovery time point upregulation of matrix metalloproteinase 9, orosomucoid 1, dishevelled-associated activator of morphogenesis 2, and arginase 1 suggest an induction in muscle damage and repair during this time frame. These results demonstrate that an acute bout of RET disrupts cellular homeostasis, induces a transient redistribution of certain leukocytes, and results in transcriptional changes in PBMCs translating into systemic changes in response to RET.
Authors:
Lara A Carlson; S W Tighe; R W Kenefick; J Dragon; N W Westcott; R J Leclair
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-03-25
Journal Detail:
Title:  European journal of applied physiology     Volume:  111     ISSN:  1439-6327     ISO Abbreviation:  Eur. J. Appl. Physiol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-17     Completed Date:  2012-06-04     Revised Date:  2014-11-16    
Medline Journal Info:
Nlm Unique ID:  100954790     Medline TA:  Eur J Appl Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2919-29     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Communication / genetics,  immunology,  physiology
Exercise / physiology*
Gene Expression Regulation
Granulocytes / immunology,  metabolism,  physiology
Humans
Inflammation / genetics,  immunology,  physiopathology
Lactic Acid / blood,  metabolism
Leukocytes, Mononuclear / immunology,  metabolism,  physiology*
Male
Microarray Analysis / methods
Resistance Training*
Rest / physiology
Signal Transduction / genetics,  immunology,  physiology
Transcription, Genetic
Young Adult
Grant Support
ID/Acronym/Agency:
P20 GM103449/GM/NIGMS NIH HHS; P20 RR016462/RR/NCRR NIH HHS; P20 RR16462/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
33X04XA5AT/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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