Document Detail


Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28.
MedLine Citation:
PMID:  14626348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the effects of centrally applied somatostatin-28 on morphometric characteristics of the thymus, the thymocyte subpopulations, as well as, on apoptosis and phases of cell cycle in thymocytes. For this purpose, peripubertal male rats were cannulated intracerebroventriculary and treated with repeated, nanomolar concentrations of somatostatin-28 (experimental group) or saline (control group). Animals were sacrificed and their thymuses were used for the analysis of thymocyte subpopulations, cell cycle and apoptosis by flow cytometry and for the evaluation of morphometric parameters by stereological analysis. Our results showed that somatostatin-28 caused decrease of the thymic mass and volume, as well as total thymocytes number. Stereological analysis revealed volume decrease of thymic cortex and medulla accompanied with cellularity decrease. Somatostatin in the deeper cortex decreased the number of thymocytes, per volume unit, while in outer cortex raised their number. A significant increase in the percentage of double-negative and both single-positive thymocyte subpopulations, in parallel with a diminished percentage of double-positive cells was found. The cellularity of double-positive and single-positive thymocyte subpopulations was decreased. Somatostatin-28 treatment augmented the percentage of apoptotic cells, while the percentage of the cells represented in phases of cell cycle was reduced. These results suggest that somatostatin-28 induce thymus hypotrophy as result of decreasing cortex and medulla volume and cellularity. Changes in the percentage and cellularity of thymocyte subpopulations and numerical density of thymocytes in outer and deeper cortex, indicate that somatostatin-28 evoked disturbance in transition of double-negative to double-positive thymocytes.
Authors:
Danica M Petrovic-Djergovic; Ana K Rakin; Irena P Zivkovic; Mileva V Micic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  34     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  2002  
Date Detail:
Created Date:  2003-11-19     Completed Date:  2004-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  573-82     Citation Subset:  IM    
Affiliation:
Immunology Research Center Branislav Jankovic, Institute of Immunology and Virology Torlak, Belgrade, Yugoslavia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Body Weight / drug effects
Cell Count
Cell Cycle
DNA / analysis
Flow Cytometry
Injections, Intraventricular
Male
Organ Size / drug effects
Protein Precursors / administration & dosage,  pharmacology*
Rats
Rats, Inbred Strains
Sexual Maturation / physiology
Somatostatin / administration & dosage,  pharmacology*
Somatostatin-28
Thymus Gland / drug effects*,  growth & development,  pathology
Chemical
Reg. No./Substance:
0/Protein Precursors; 51110-01-1/Somatostatin; 75037-27-3/Somatostatin-28; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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