Document Detail


Changes in the susceptibility of red blood cells to oxidative and osmotic stress following submaximal exercise.
MedLine Citation:
PMID:  7671878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Red blood cell (RBC) susceptibility to oxidative and osmotic stress in vitro was investigated in cells from trained and untrained men before and after submaximal exercise. Whilst no significant change in peroxidative haemolysis occurred immediately after 1 h of cycling at 60% of maximal aerobic capacity (VO2max), a 20% increase was found 6h later in both groups (P < 0.05). The RBC osmotic fragility decreased by 15% immediately after exercise (P < 0.001) and this was maintained for 6h (P < 0.001). There was an associated decrease in mean cell volume (P < 0.05). Training decreased RBC susceptibility to peroxidative haemolysis (P < 0.025) but it did not influence any other parameter. These exercise-induced changes were smaller in magnitude but qualitatively similar to those found in haemopathological states involving haem-iron incorporation into membrane lipids and the short-circuiting of antioxidant protection. To explore this similarity, a more strenuous and mechanically stressful exercise test was used. Running at 75% VO2max for 45 min reduced the induction time of O2 uptake (peroxidation), consistent with reduced antioxidation capacity, and increased the maximal rate of O2 uptake in RBC challenged with cumene hydroperoxide (P < 0.001). The proportion of high-density RBC increased by 10% immediately after running (P < 0.001) but no change in membrane-incorporated haem-iron occurred. In contrast, treatment of RBC with oxidants (20-50 mumol.l-1) in vitro increased cell density and membrane incorporation of haem-iron substantially. These results showed that single episodes of submaximal exercise caused significant changes in RBC susceptibility to oxidative and osmotic stress. Such responses may account for the increase in RBC turnover found in athletes undertaking strenuous endurance training.
Authors:
J A Smith; M Kolbuch-Braddon; I Gillam; R D Telford; M J Weidemann
Related Documents :
7039688 - Effects of whole-body gamma irradiation on oxygen transport by rat erythrocytes.
3797628 - Role of water and ethanol radicals in the radiation destruction of human deoxyhemoglobin.
719168 - Variability of the homeostatic response to altered p50.
15531998 - Is hemoglobin desaturation related to blood viscosity in athletes during exercise?
10562618 - Skeletal muscle metabolism during high-intensity sprint exercise is unaffected by dichl...
6809458 - The consistency of cardiac output measurement (co2 rebreathe) in children during exercise.
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of applied physiology and occupational physiology     Volume:  70     ISSN:  0301-5548     ISO Abbreviation:  Eur J Appl Physiol Occup Physiol     Publication Date:  1995  
Date Detail:
Created Date:  1995-10-16     Completed Date:  1995-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410266     Medline TA:  Eur J Appl Physiol Occup Physiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  427-36     Citation Subset:  IM    
Affiliation:
Division of Biochemistry and Molecular Biology, School of Life Sciences, Faculty of Science, Australian National University, Canberra.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Bicycling
Chemiluminescent Measurements
Erythrocytes / physiology*
Exercise / physiology*
Heme / metabolism
Humans
Lipid Peroxidation / physiology
Male
Osmotic Fragility / physiology
Oxidative Stress / physiology*
Oxygen Consumption / physiology
Physical Education and Training
Pilot Projects
Running
Chemical
Reg. No./Substance:
14875-96-8/Heme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Force-length, torque-angle and EMG-joint angle relationships of the human in vivo biceps brachii.
Next Document:  Meal size and frequency: effect on potentiation of the thermal effect of food by prior exercise.