Document Detail

Changes in sulfated proteoglycan production after activation of rat liver macrophages.
MedLine Citation:
PMID:  1860687     Owner:  NLM     Status:  MEDLINE    
Production of extracellular matrix proteins-in particular, the proteoglycans-by macrophages is important in many of their functions, including cell-cell recognition, adhesion and phagocytosis. In this study, we characterized changes in sulfated proteoglycan production by hepatic macrophages following in vivo activation with lipopolysaccharide. We found that both resident Kupffer cells and liver macrophages from lipopolysaccharide-treated rats incorporated [35S]sulfate into proteoglycans. Lipopolysaccharide-activated macrophages incorporated two to three times more of the label than did resident Kupffer cells. In addition, although both cell types produced chondroitin sulfate and heparan sulfate, resident Kupffer cells synthesized more chondroitin sulfate whereas lipopolysaccharide-activated cells produced more heparan sulfate. Using specific antibodies and flow cytometry, we also found that hepatic macrophages produced chondroitin-4-sulfate, chondroitin-6-sulfate and chondroitin-O-sulfate. Lipopolysaccharide-activated macrophages contained more chondroitin-4-sulfate and chondroitin-O-sulfate and less heparan sulfate than did resident Kupffer cells. Both tunicamycin and beta-D-xylosides, inhibitors of sulfated proteoglycan biosynthesis, were found to block phagocytosis by the cells. Taken together, these results suggest that sulfated proteoglycans are important in activation and functional responsiveness of liver macrophages.
J D Laskin; A Dokidis; C R Gardner; D L Laskin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  14     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-08-30     Completed Date:  1991-08-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  306-12     Citation Subset:  IM    
Department of Environmental and Community Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
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MeSH Terms
Flow Cytometry
Liver / cytology,  metabolism*
Macrophages / metabolism*,  physiology
Phagocytosis / drug effects
Proteoglycans / biosynthesis*
Tunicamycin / pharmacology
Grant Support
Reg. No./Substance:
0/Proteoglycans; 11089-65-9/Tunicamycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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