Document Detail


Changes in the subcellular distribution of free carnitine and its acyl derivatives in diabetic rat hearts following treatment with L-carnitine.
MedLine Citation:
PMID:  8164344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carnitine deficiency has been demonstrated in diabetic hearts, and it is also well known that L-carnitine administration has a beneficial effect on cardiac function. Carnitine treatment would be expected to reduce the accumulation of long-chain acylcarnitine. However, many reports have shown that myocardial long-chain acylcarnitine levels were increased following treatment with L-carnitine in whole-heart studies. Since acylcarnitine exists in both the mitochondrial and cytosolic compartments, it is difficult to investigate changes in subcellular distribution by studying whole-heart preparations. The present study investigated the myocardial subcellular distribution of carnitine and its acyl derivatives in diabetic rats with or without L-carnitine treatment. Approximately 90% of total cellular carnitine was located in the cytosol in the diabetic hearts. Both mitochondrial and cytosolic levels of free carnitine and short-chain acylcarnitine were significantly decreased in the diabetic heart. However, the mitochondrial level of long-chain acylcarnitine was significantly increased. L-carnitine treatment reduced the mitochondrial level of long-chain acylcarnitine, but the cytosolic level of long-chain acylcarnitine was significantly increased. These results show that L-carnitine treatment prevents the accumulation of long-chain acylcarnitine in the mitochondrial space and then reduces the detergent effect of long-chain acylcarnitine on the mitochondrial membrane. We suggest that it is a possible mechanism of the beneficial effect of L-carnitine treatment on the diabetic heart.
Authors:
S Aoshima; S Fujisawa; A Kobayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese heart journal     Volume:  34     ISSN:  0021-4868     ISO Abbreviation:  Jpn Heart J     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-05-20     Completed Date:  1994-05-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401175     Medline TA:  Jpn Heart J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  763-72     Citation Subset:  IM    
Affiliation:
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carnitine / analogs & derivatives,  metabolism*,  pharmacology*
Cytosol / metabolism
Diabetes Mellitus, Experimental / metabolism*
Male
Mitochondria, Heart / metabolism
Myocardium / metabolism*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
541-15-1/Carnitine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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