| Changes in somatostatin receptor expression of the pancreas and effectiveness of octreotide in rats with acute necrotizing pancreatitis. | |
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MedLine Citation:
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PMID: 15612670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate changes in the expression of the somatostatin receptor (SSTR) of the pancreas and of pancreatic blood flow, and its relationship to the metabolism of eicosanoids in order to elucidate the effectiveness of octreotide, an analog of somatostatin, in acute necrotizing pancreatitis (ANP). METHODS: A model of ANP was induced in rats with injection of sodium taurocholate via the pancreaticobiliary duct. The SSTR was detected using a radioligand binding assay (RBA) with 125I-somatostatin-14, and the SSTR2 mRNA of the pancreas was analyzed using in situ hybridization. Pancreatic blood flow and the metabolites of eicosanoids were also determined. RESULTS: The SSTR of the pancreas was 109.70 +/- 58.32 fmol/mg protein in normal rats. A significant decrease in the SSTR, together with the signals of SSTR2 mRNA, was shown at 3, 6 and 12 h after onset of ANP. Pancreatic blood flow was reduced and thromboxin-2 was increased significantly in the course of ANP. In the ANP group treated with octreotide, both the decrease in pancreatic blood flow and the abnormal metabolism of eicosanoids were corrected, and the pathological damage was relieved. CONCLUSION: SSTR expression of the pancreas is significantly reduced in ANP. Correction of the abnormal metabolism of eicosanoids and improvement in pancreatic microcirculation may be the major mechanism of somatostatin analogs in the treatment of ANP and inhibition of pancreatic enzymes via their receptors plays a minor role. |
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Authors:
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Jian Xin Wu; Yao Zong Yuan; Jia Yu Xu; Zong Qin Xia; Lan Feng Qin; Zheng Lin Zheng; Ding Guo Li; Han Ming Lu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chinese journal of digestive diseases Volume: 5 ISSN: 1443-9611 ISO Abbreviation: Chin J Dig Dis Publication Date: 2004 |
Date Detail:
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Created Date: 2004-12-22 Completed Date: 2005-01-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101088612 Medline TA: Chin J Dig Dis Country: Australia |
Other Details:
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Languages: eng Pagination: 35-9 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Xinhua Hospital, Shanghai Second Medical University, Shanghai, China. wjxgp@public9.sta.net.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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6-Ketoprostaglandin F1 alpha
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blood Animals Dinoprostone / blood Gastrointestinal Agents / therapeutic use* Male Octreotide / therapeutic use* Pancreas / blood supply, metabolism Pancreatitis, Acute Necrotizing / drug therapy, metabolism*, physiopathology Rats Rats, Sprague-Dawley Receptors, Somatostatin / metabolism* Thromboxane B2 / blood |
| Chemical | |
Reg. No./Substance:
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0/Gastrointestinal Agents; 0/Receptors, Somatostatin; 363-24-6/Dinoprostone; 54397-85-2/Thromboxane B2; 58962-34-8/6-Ketoprostaglandin F1 alpha; 83150-76-9/Octreotide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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