Document Detail


Changes in signalling molecule levels in 10-day hindlimb immobilized rat muscles.
MedLine Citation:
PMID:  15676058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: Hindlimb immobilization produces similar percentage decreases in muscle mass in the predominantly type I soleus and type II vastus lateralis muscles. Consequently we hypothesized that the percentage changes in potential regulatory molecules for atrophy would be similar in the two muscle fibre types. METHODS: Therefore, the purpose of the current study was to measure phosphorylated p38 MAPK and JNK, as well as the protein levels of p53, growth arrest and DNA damage-inducible 45 (GADD45), and full-length poly(ADP-ribose) polymerase (PARP) to determine whether their changes in expression in the soleus and vastus lateralis muscles were similar at 10th day of hindlimb immobilization in young rats. RESULTS: Unexpectedly, preferential increases in phosphorylation of p38 MAPK and JNK and in the protein levels of p53, GADD45, as well as decreases in full-length PARP occurred in the soleus muscle, while only p38 phosphorylation increased in the white portion of the vastus lateralis muscle at 10th day of hindlimb immobilization. CONCLUSION: Taken together, these results are interpreted to suggest that some of regulatory processes or kinetics in the atrophy of type I and II muscle fibres during limb immobilization may differ at the 10th day of limb immobilization.
Authors:
S Machida; F W Booth
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  183     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-28     Completed Date:  2005-03-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  England    
Other Details:
Languages:  eng     Pagination:  171-9     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA Damage
Hindlimb
Immobilization
Intracellular Signaling Peptides and Proteins
JNK Mitogen-Activated Protein Kinases / analysis
Male
Mitogen-Activated Protein Kinases / analysis*
Muscle Proteins / analysis*
Muscle, Skeletal / chemistry*,  enzymology,  growth & development
Muscular Atrophy / metabolism
Phosphorylation
Poly(ADP-ribose) Polymerases / analysis
Proteins / analysis
Rats
Rats, Inbred F344
Tumor Suppressor Protein p53 / analysis
p38 Mitogen-Activated Protein Kinases / analysis
Grant Support
ID/Acronym/Agency:
AR19393/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/GADD45 protein; 0/Intracellular Signaling Peptides and Proteins; 0/Muscle Proteins; 0/Proteins; 0/Tumor Suppressor Protein p53; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

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