Document Detail

Changes in secretory cells of hamster tracheal epithelium in response to acute sublethal injury: a quantitative study.
MedLine Citation:
PMID:  6552208     Owner:  NLM     Status:  MEDLINE    
Secretory cells are reported to rapidly increase in number when the tracheobronchial epithelium is irritated. We suggest that this acute change results from accumulation of secretion granules within specialized albeit unobstrusive secretory cells rather than from conversion of undifferentiated cells to secretory cells of the production of new cells by mitosis. This hypothesis was tested in hamster tracheal epithelium 6, 12, and 24 hr after intratracheal instillation of elastase in saline or saline alone. Basal, secretory, and ciliated cells and cells of indeterminate character were quantified by counting 1400 cells per hamster in 2 micrometers thick glycol methacrylate sections stained with periodic acid-Schiff (PAS)-lead hematoxylin. Secretory cells were characterized and quantified depending upon distribution patterns of the PAS-positive secretion granules and on the amount of intracellular secretion product. Thirty-two hamsters were studied; half of them received colchicine 6 hr prior to tissue sampling. Over 24 hr the percentage of ciliated cells showed no significant change and no morphological evidence of alteration was observed in this cell population; thus ciliated cells were excluded from the analysis. When the ciliated cell population was excluded, control and experimental values for the different cell categories remained statistically constant, if unavoidable classification errors at the light microscopic level were compensated for on the basis of ultrastructural features. Average control values for the non-ciliated cell population were: mitotic index 0.030, basal cells 27.8%, secretory cells 57.9%, and cells of indeterminate character 14.3%. Secretory product was decreased in secretory cells at 6 hr but thereafter secretion accumulated in these cells. At 24 hr the overall number of secretory cells appeared to be increased. However, the increase was apparent and not real and was accounted for by accumulation of secretion granules in preexisting but previously unobtrusive secretory cells.
E M McDowell; J W Combs; C Newkirk
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental lung research     Volume:  4     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  227-43     Citation Subset:  IM    
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MeSH Terms
Colchicine / pharmacology
Cytoplasmic Granules / drug effects
Epithelial Cells
Microscopy, Electron
Pancreatic Elastase / pharmacology*
Periodic Acid-Schiff Reaction
Trachea / cytology,  drug effects,  secretion*
Grant Support
Reg. No./Substance:
0/lead haematoxylin; 517-28-2/Hematoxylin; 64-86-8/Colchicine; EC Elastase

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