| Changes in rat papilla microsomal membrane fluidity during development. | |
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MedLine Citation:
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PMID: 10095144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During maturation, rat renal papillary microsomes suffer a rearrangement in their fatty acid phospholipid composition. The most significant changes in total phospholipids are the increase in their content of the 20:4 and a decrease in the levels of 14:0, 16:0, 18:1, 22:6 and 20:3 fatty acids. The changes in total phospholipid fatty acid content are a reflection of the variations in the individual phospholipid composition. During this period, microsomal cholesterol, phospholipid, and protein concentrations present no variations. Steady state fluorescence anisotropy obtained by using TMA-DPH (see text) as a fluorescence probe denoted higher values for 70- versus 10-day-old microsomes. Using DPH as a probe, steady state fluorescence anisotropy was determined in whole microsomes, as well as in total lipid and phospholipid vesicles, from both 10- and 70-day-old papillary cells. No differences were detected in phospholipid and total lipid vesicles between days 10 and 70. On the other hand, 10-day-old microsomes appeared to be less fluid than adult microsomes. The results indicate that these structural changes in kidney membranes during development might affect protein-lipid interaction and, therefore, the activity of many membrane enzymes. |
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Authors:
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C L D'Antuono; N B Sterin-Speziale |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of the neonate Volume: 75 ISSN: 0006-3126 ISO Abbreviation: Biol. Neonate Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-05-27 Completed Date: 1999-05-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0247551 Medline TA: Biol Neonate Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 300-9 Citation Subset: IM |
Affiliation:
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Instituto de Química y Fisicoquímica Biológica (IQUIFIB), Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, UBA-CONICET, Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diphenylhexatriene / analogs & derivatives Fluorescence Polarization Fluorescent Dyes Intracellular Membranes / physiology* Kidney Medulla / growth & development*, ultrastructure* Membrane Fluidity* Microsomes / ultrastructure* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 1720-32-7/Diphenylhexatriene; 71316-28-4/1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene |
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