Document Detail


Changes in production of ethanol, acids and H2 from glucose by the fecal flora of a 16- to 158-d-old breast-fed infant.
MedLine Citation:
PMID:  9430607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microbes in the adult human colon ferment dietary substrates chiefly to acetic, propionic and butyric acids and CO2, H2 and CH4. How this fermentation evolves after microbial colonization of the neonate is unknown. We examined the fermentation of glucose by fecal suspensions of a breast-fed infant from d 16 to 158 and found that the fermentation changed with age. Acetate, ethanol, succinate, lactate, formate and H2 were formed up to 117 d of age. Production of succinate, lactate, formate and H2 ceased after 117 d and acetate production increased. Butyrate and propionate were minor products up to 117 d. Afterwards, there was a slight increase in propionate production with no change in butyrate formation. Acetate was always the major product of glucose fermentation by the fecal suspensions. Approximately the same amounts of ethanol were formed throughout the study period. The fermentations were similar to fermentations of Escherichia coli and streptococci through 117 d. Nuclear magnetic resonance (NMR) analysis of the acetate formed from 1-13C- and 3-13C-glucose showed that the dominant fermentation pathway used by the colonic microbes switched from the Embden-Meyerhof-Parnas pathway at 16 d of age to the Bifidobacterium pathway at 158 d of age. An increase in the contribution of the Bifidobacterium fermentation to the overall colonic fermentation after 117 d would account for the increase in the formation of acetate from glucose. Chemical and NMR analyses of products of fecal fermentations from two other breast-fed infants <1 mo old were similar to those of the infant examined between 16 and 158 d.
Authors:
M J Wolin; S Yerry; T L Miller; Y Zhang; S Bank
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  128     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-01-29     Completed Date:  1998-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  85-90     Citation Subset:  IM    
Affiliation:
Wadsworth Center for Laboratories and Research, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Aging
Breast Feeding*
Carboxylic Acids / metabolism*
Chromatography, High Pressure Liquid
Ethanol / metabolism*
Feces / microbiology*
Fermentation
Formic Acids / metabolism
Glucose / metabolism*
Humans
Hydrogen / metabolism*
Infant
Infant, Newborn
Lactic Acid / metabolism
Lactose / metabolism
Magnetic Resonance Spectroscopy
Succinic Acid / metabolism
Chemical
Reg. No./Substance:
0/Acetates; 0/Carboxylic Acids; 0/Formic Acids; 110-15-6/Succinic Acid; 1333-74-0/Hydrogen; 50-21-5/Lactic Acid; 50-99-7/Glucose; 63-42-3/Lactose; 64-17-5/Ethanol; 64-18-6/formic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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