| Changes in polypeptide synthesis and glycosylation in mouse embryonic fibroblast C3H/10T1/2 Cl 8 cells caused by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate. | |
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MedLine Citation:
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PMID: 3790579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of tumor promotion is not well understood. We have used the transformable, tumor promotable, mouse embryo fibroblast C3H/10T1/2 Cl 8 cells to study tumor promoter specific changes in protein synthesis and protein glycosylation. Two-dimensional gel electrophoresis showed that 12-O-tetradecanoylphorbol 13-acetate caused a significant increase in the synthesis of five cellular and 34 extracellular polypeptides. One of these polypeptides has tentatively been identified as ornithine decarboxylase. One new polypeptide (p 62, Mr 58,000) was found in the medium of 12-O-tetradecanoylphorbol 13-acetate-treated cells. The amounts of several excreted proteins were enhanced 5-10 fold by 12-O-tetradecanoylphorbol 13-acetate. 12-O-tetradecanoylphorbol 13-acetate interfered with glycosylation both by affecting protein synthesis and also directly with glycosylation. At least 15 polypeptides in the medium and two cellular polypeptides decreased after 12-O-tetradecanoylphorbol 13-acetate treatment. Two of the major polypeptides found in the medium (p 8 and 10, Mr approx. 200,000-220,000) have properties similar to fibronectin, while p 9 and 11 both found in the cellular preparations and in the medium (Mr 180,000 and 150,000) were collagenase sensitive and their synthesis was inhibited by 12-O-tetradecanoylphorbol 13-acetate. |
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Authors:
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L Haarr; K Kleppe; J R Lillehaug |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 889 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1986 Dec |
Date Detail:
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Created Date: 1987-02-02 Completed Date: 1987-02-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 334-45 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Transformation, Neoplastic / chemically induced*, metabolism Embryo, Mammalian Fibroblasts / drug effects, metabolism* Gene Expression Regulation / drug effects Glycoproteins / biosynthesis* Mice Mice, Inbred C3H Neoplasm Proteins / biosynthesis* Peptide Biosynthesis* Protein Processing, Post-Translational / drug effects Tetradecanoylphorbol Acetate / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Neoplasm Proteins; 16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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