Document Detail

Changes in polypeptide synthesis and glycosylation in mouse embryonic fibroblast C3H/10T1/2 Cl 8 cells caused by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate.
MedLine Citation:
PMID:  3790579     Owner:  NLM     Status:  MEDLINE    
The mechanism of tumor promotion is not well understood. We have used the transformable, tumor promotable, mouse embryo fibroblast C3H/10T1/2 Cl 8 cells to study tumor promoter specific changes in protein synthesis and protein glycosylation. Two-dimensional gel electrophoresis showed that 12-O-tetradecanoylphorbol 13-acetate caused a significant increase in the synthesis of five cellular and 34 extracellular polypeptides. One of these polypeptides has tentatively been identified as ornithine decarboxylase. One new polypeptide (p 62, Mr 58,000) was found in the medium of 12-O-tetradecanoylphorbol 13-acetate-treated cells. The amounts of several excreted proteins were enhanced 5-10 fold by 12-O-tetradecanoylphorbol 13-acetate. 12-O-tetradecanoylphorbol 13-acetate interfered with glycosylation both by affecting protein synthesis and also directly with glycosylation. At least 15 polypeptides in the medium and two cellular polypeptides decreased after 12-O-tetradecanoylphorbol 13-acetate treatment. Two of the major polypeptides found in the medium (p 8 and 10, Mr approx. 200,000-220,000) have properties similar to fibronectin, while p 9 and 11 both found in the cellular preparations and in the medium (Mr 180,000 and 150,000) were collagenase sensitive and their synthesis was inhibited by 12-O-tetradecanoylphorbol 13-acetate.
L Haarr; K Kleppe; J R Lillehaug
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  889     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-02-02     Completed Date:  1987-02-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  334-45     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Transformation, Neoplastic / chemically induced*,  metabolism
Embryo, Mammalian
Fibroblasts / drug effects,  metabolism*
Gene Expression Regulation / drug effects
Glycoproteins / biosynthesis*
Mice, Inbred C3H
Neoplasm Proteins / biosynthesis*
Peptide Biosynthesis*
Protein Processing, Post-Translational / drug effects
Tetradecanoylphorbol Acetate / pharmacology*
Reg. No./Substance:
0/Glycoproteins; 0/Neoplasm Proteins; 16561-29-8/Tetradecanoylphorbol Acetate

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