| Changes in plant mitochondrial electron transport alter cellular levels of reactive oxygen species and susceptibility to cell death signaling molecules. | |
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MedLine Citation:
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PMID: 17012741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transgenic tobacco (Nicotiana tabacum) lacking mitochondrial alternative oxidase (AOX) have been compared with wild-type (Wt) tobacco using two different systems, either suspension cell cultures or leaves. In both systems, a lack of AOX was accompanied by an increase in some anti-oxidant defenses, consistent with the hypothesis that a lack of AOX increases the mitochondrial generation of reactive oxygen species (ROS). In most cases, this increase in anti-oxidant defenses could more than offset the presumed increased rate of ROS generation, resulting paradoxically in a lower steady-state level of ROS than was found in Wt leaves or suspension cells. We also found that the amount of cell death induced by salicylic acid or nitric oxide correlated strongly with the level of ROS (irrespective of the level of AOX), while death induced by azide was dependent upon the presence or absence of AOX. These results suggest that susceptibility to cell death by signaling molecules (salicylic acid and nitric oxide) is dependent upon the steady-state cellular level of ROS and that AOX levels clearly contribute to this steady state, perhaps by influencing the rate of mitochondrial-generated ROS and hence the cellular level of anti-oxidant defenses. |
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Authors:
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Sasan Amirsadeghi; Christine A Robson; Allison E McDonald; Greg C Vanlerberghe |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-30 |
Journal Detail:
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Title: Plant & cell physiology Volume: 47 ISSN: 0032-0781 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-23 Completed Date: 2007-02-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 1509-19 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada M1C1A4. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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metabolism Cell Death / drug effects Electron Transport / drug effects* Electron Transport Complex IV / metabolism Gene Expression Regulation, Plant / drug effects Genes, Plant Mitochondria / drug effects, metabolism* Nitric Oxide / pharmacology* Oxidoreductases / deficiency Plant Leaves / drug effects Plant Proteins / metabolism Plants, Genetically Modified RNA, Messenger / genetics, metabolism Reactive Oxygen Species / metabolism* Salicylic Acid / pharmacology* Tobacco / cytology, drug effects, metabolism* Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Plant Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide; 69-72-7/Salicylic Acid; EC 1.-/Oxidoreductases; EC 1.-/alternative oxidase; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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