Document Detail


Changes in the pattern of androgen formation in vitro by the baboon fetal adrenal gland at mid- and late gestation.
MedLine Citation:
PMID:  2964875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, baboon fetal adrenal cells were obtained at mid- and late gestation and incubated for various intervals to determine simultaneously the effects of length of incubation and stage of development on the pattern of adrenal steroidogenesis. Cells were treated with adrenocorticotropic hormone (ACTH) from 0 to 48 h of incubation, and the concentrations of dehydroepiandrosterone (DHA), DHA-sulfate (DHAS), and androstenedione (delta 4A) were determined in the medium. The secretion of DHA and DHAS by untreated or ACTH-treated cells of midgestation increased linearly throughout the 48-h incubation period. In fetal adrenal incubates of late gestation, however, DHA and DHAS concentrations peaked at 3 h and declined thereafter, suggesting that the DHA secreted into the medium was further metabolized by this tissue. Baboon fetal adrenal cells formed similar amounts of DHAS and DHA at midgestation, but greater quantities of DHAS were formed at term. In fetal adrenal incubates of midgestation, DHA concentrations exceeded those of delta 4A by threefold, a relationship which was reversed at late gestation, probably due to the increase in the activity of 3 beta-hydroxy-steroid dehydrogenase with advancing gestation. Because the decline in DHA with time of incubation was also associated with a concomitant decrease in DHAS and no change in delta 4A, it does not appear that formation of these steroids account for the loss of DHA. We conclude that the pattern of androgen metabolism exhibited by fetal adrenal cells obtained at midgestation is different from that at term.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M L Walker; G J Pepe; E D Albrecht
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  37     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-05-03     Completed Date:  1988-05-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1192-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / embryology,  metabolism*
Androstenedione / biosynthesis*,  secretion
Animals
Body Weight
Dehydroepiandrosterone / analogs & derivatives*,  biosynthesis*,  secretion
Dehydroepiandrosterone Sulfate
Female
Fetus / metabolism
Gestational Age
Male
Organ Size
Papio
Pregnancy
Radioimmunoassay
Grant Support
ID/Acronym/Agency:
F32 HD06903/HD/NICHD NIH HHS; R01 HD13294/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
53-43-0/Dehydroepiandrosterone; 63-05-8/Androstenedione; 651-48-9/Dehydroepiandrosterone Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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