Document Detail

Changes in oncogene expression in ascite tumour cells during ageing.
MedLine Citation:
PMID:  10465014     Owner:  NLM     Status:  MEDLINE    
The expression of two oncogenes, c-myc and c-fos, was studied in an ascitic tumour (ATPC+) at different times after implantation. The specific mRNA synthesis was analysed by Northern blot analysis. The presence of the oncogene proteins was shown by immunofluorescence using flow cytometry and referred to the distribution of the cells in the different cell phases. The results show that both oncogenes are expressed by ATPC+ tumour cells. c-myc is expressed 5, 8 and 12 days after implantation, although with a different intensity, and the protein is mainly present in S or S+G2 phase cells. The c-fos oncogene is expressed only 12 days after tumour implantation and the cells labelled with the specific antibody are mainly in G1 phase. We conclude that c-myc is principally correlated with proliferative activity, whereas c-fos is expressed by non-cycling cells.
M Biagetti; M A Della Fazia; G Servillo; M P Viola-Magni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell proliferation     Volume:  27     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-200     Citation Subset:  IM    
Institute of General Pathology, University of Perugia, Italy.
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MeSH Terms
Ascites / physiopathology
Cell Aging / physiology
Cell Count
Cell Cycle / physiology
DNA / metabolism
Gene Expression / physiology*
Macrophages / pathology
Mice, Inbred BALB C
Oncogenes / genetics*
Proliferating Cell Nuclear Antigen / metabolism
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-myc / genetics
RNA, Messenger / metabolism
Tumor Cells, Cultured / metabolism,  pathology,  physiology*
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 9007-49-2/DNA

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