Document Detail

Changes in neurotransmitter turnover in locus coeruleus produced by changes in arterial blood pressure.
MedLine Citation:
PMID:  2461245     Owner:  NLM     Status:  MEDLINE    
The effect of drug-induced hypertension and hypotension on neurotransmitter metabolism in the locus coeruleus (LC) of urethane anesthetized rats was studied using in vivo electrochemical methods. Peaks were seen at +0.15 V and +0.28 V. Studies with alpha-methylparatyrosine, fusaric acid and pargyline showed the first peak was produced by extracellular fluid dihydroxyphenylacetic acid (DOPAC) while the second peak was 5-hydroxyindoleacetic acid (5-HIAA). Phenylephrine was infused intravenously to raise the blood pressure by 50 mmHg, nitroprusside IV was used to reduce the blood pressure by 20 mmHg. During phenylephrine hypertension, the electrochemical signal for DOPAC showed an initial small reduction followed by a later significant increase which persisted even after the infusion was stopped. The signal for 5-HIAA rose with the onset of hypertension and remained elevated. Nitroprusside hypotension did not change the DOPAC peak but did lead to an immediate and persistent increase in the electrochemical 5-HIAA peak. To confirm the electrochemical findings, other groups of rats were decapitated during and after hypertensive and hypotensive drug infusions and the LC was assayed for norepinephrine, dopamine, DOPAC, serotonin, and 5-HIAA using HPLC with electrochemical detection. Changes in tissue DOPAC and 5-HIAA concentrations supported the electrochemical electrode observations. The effect of clonidine on the electrochemical recordings from LC was also studied. Clonidine reduced the catechol peak. No change was observed in the 5-HIAA peak during the infusion, but the 5-HIAA peak went up after the infusion was stopped. These experiments show that hypertension, hypotension, and alpha-2 agonists lead to changes in catecholamine and indoleamine metabolism in LC.
D Bhaskaran; C R Freed
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research bulletin     Volume:  21     ISSN:  0361-9230     ISO Abbreviation:  Brain Res. Bull.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-12-27     Completed Date:  1988-12-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7605818     Medline TA:  Brain Res Bull     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-9     Citation Subset:  IM    
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / metabolism
Blood Pressure* / drug effects
Hydroxyindoleacetic Acid / metabolism
Hypertension / metabolism*
Hypotension / metabolism*
Locus Coeruleus / metabolism*
Neurotransmitter Agents / metabolism*
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Neurotransmitter Agents; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 54-16-0/Hydroxyindoleacetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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