| Changes in myofilament proteins, but not calcium regulation, are associated with a high fat diet-induced improvement in contractile function in heart failure. | |
| | |
MedLine Citation:
|
PMID: 21765056 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Pathological conditions such as diabetes, insulin resistance, and obesity are characterized by elevations in plasma and myocardial lipids, and are reported to exacerbate the progression of heart failure (HF). Alterations in cardiomyocyte calcium (Ca(2+)) regulatory properties and myofilament proteins are also implicated in contractile dysfunction in HF. However, our prior studies reported that high saturated fat feeding improves in vivo myocardial contractile function, thereby exerting a cardioprotective effect in HF. Therefore, we hypothesize that high saturated fat feeding improves contractile function by altering Ca(2+) regulatory properties and myofilament protein expression in HF. Male Wistar rats underwent coronary artery ligation or sham surgery (SH) and were fed normal chow (SHNC and HFNC) or high saturated fat diet (SHSAT and HFSAT) for 8 weeks. Contractile properties were measured in vivo [echocardiography and left ventricular (LV) cannulation] and in isolated LV cardiomyocytes. in vivo measures of contractility (peak LV ± dP/dt) were depressed in HFNC vs. SHNC but improved in HFSAT. Isolated cardiomyocytes from both HF groups were hypertrophied, had decreased % cell shortening and prolonged time to half decay of Ca(2+) transient vs. SH; however, high fat reduced in vivo myocyte hypertrophy in HFSAT only. Peak velocity of cell shortening, -dL/dt, was reduced in HFNC, but not HFSAT and was positively correlated with in vivo contractile function (peak LV +dP/dt). HFNC demonstrated a myosin heavy chain (MHC) isoform switch from fast MHCα to slow MHCβ that was prevented in HFSAT. Alterations in Ca(2+) transients, L-type Ca(2+) currents and protein expression of sarcoplasmic reticulum (SR) Ca(2+) ATPase and phosphorylated phospholamban could not account for changes in in vivo contractile properties. In conclusion, the cardioprotective effect associated with high saturated fat feeding in HF may occur at the level of the isolated cardiomyocyte, specifically involving changes in myofilament function, but not SR Ca(2+) regulatory properties. |
| | |
Authors:
|
Yi-Hsin F Cheng; Wei Li; Tracy A McElfresh; Xiaoqin Chen; Jessica M Berthiaume; Laurie Castel; Xin Yu; David R Van Wagoner; Margaret P Chandler |
Related Documents
:
|
19545596 - Gingival vascular damage in atherosclerotic rabbits: hydroxytyrosol and squalene benefits. 1466646 - Probucol reduces plasma and aortic wall oxysterol levels in cholesterol fed rabbits ind... 7400686 - Effects of dietary protein on turnover, oxidation, and absorption of cholesterol, and o... 7616126 - Tissue specific changes in acyl-coa: cholesterol acyltransferase (acat) mrna levels in ... 19115316 - Vsl#3 probiotic treatment attenuates fibrosis without changes in steatohepatitis in a d... 8349496 - Body dimensions and carcass measurements of cattle selected for postweaning gain fed tw... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-15 |
Journal Detail:
|
Title: American journal of physiology. Heart and circulatory physiology Volume: - ISSN: 1522-1539 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1Case Western Reserve University. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of increased systolic Ca2+ and phospholamban phosphorylation during {beta}-adrenergic stimul...
Next Document: Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced infl...