Document Detail


Changes in molecular weight of hyaluronan and hyaluronidase activity in uterine cervical mucus in cervical ripening.
MedLine Citation:
PMID:  11380283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: This study was undertaken to investigate changes in the forms of hyaluronan and hyaluronidase activity in cervical mucus during cervical ripening. METHODS: Uterine cervical mucus was obtained from 57 pregnant women (25 at preterm gestation, ten at term gestation, 11 within 1 week before labor, and 11 during the first stage of labor). We determined 1) concentration of hyaluronan, 2) hyaluronidase activity, and 3) molecular weight of hyaluronan in cervical mucus. Data are presented as mean and range. RESULTS: The hyaluronan concentration in mucus in the 1st stage of labor (1.58 microg/ml, 0.46-23.96) was significantly (p<0.05) higher than that in all other groups (preterm: 0.29, 0.10-0.88; term: 0.24, 0.11-0.80; within 1 week before labor: 0.30, 0.18-0.62). Hyaluronidase activity both within 1 week before labor group (3.03 min., 1.12-3.95) and in 1st stage of labor group (3.52, 0.43-5.15) was significantly (p<0.05) higher than that in preterm group (1.70, 0.00-5.47). The molecular weight of hyaluronan in cervical mucus in the 1st stage of labor (0.97x106, 0.86-1.41) was significantly (p<0.05) lower than in the preterm and term groups (preterm: 1.60, 1.21-2.20, term: 1.41, 1.21-2.20). There was a significant correlation between hyaluronidase activity and molecular weight of hyaluronan (p<0.05, r=-0.41, n=23). CONCLUSION: These findings suggest that either hyaluronidase or low-molecular weight hyaluronan could be one of the most important regulators in the process of cervical ripening.
Authors:
M Obara; H Hirano; M Ogawa; H Tsubaki; N Hosoya; Y Yoshida; S Miyauchi; T Tanaka
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta obstetricia et gynecologica Scandinavica     Volume:  80     ISSN:  0001-6349     ISO Abbreviation:  Acta Obstet Gynecol Scand     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-30     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370343     Medline TA:  Acta Obstet Gynecol Scand     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  492-6     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Akita University School of Medicine, Akita, Japan.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Biological Markers / analysis
Cervical Ripening / metabolism,  physiology*
Cervix Mucus / chemistry*,  enzymology*
Chromatography, High Pressure Liquid
Female
Gestational Age
Humans
Hyaluronic Acid / analysis*,  metabolism
Hyaluronoglucosaminidase / analysis*,  metabolism
Labor Stage, First
Labor Stage, Third
Molecular Weight
Pregnancy
Probability
Prospective Studies
Sensitivity and Specificity
Statistics, Nonparametric
Chemical
Reg. No./Substance:
0/Biological Markers; 9004-61-9/Hyaluronic Acid; EC 3.2.1.35/Hyaluronoglucosaminidase

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