Document Detail


Changes in metaphasic chromosomal ultrastructure caused by denaturing treatments.
MedLine Citation:
PMID:  3075165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Denaturation and posterior renaturation of metaphase chromosomes after different treatments were tested by acridine orange staining. A relationship between conservation of ultrastructure and the capacity to renature was discovered. Amyl acetate, commonly used in electron microscopy to fix chromosomes, avoids the action of denaturing agents and this effect is not reversed by chromosome rehydration.
Authors:
E Cerdán; J Pasantes; J Méndez; V Goyanes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  55     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1988  
Date Detail:
Created Date:  1989-08-02     Completed Date:  1989-08-02     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  167-70     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica, Colegio Universitario de La Coruña, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chromosomes / drug effects,  ultrastructure*
Cricetinae
Cricetulus
Formamides / pharmacology
Hydrochloric Acid / pharmacology
Metaphase*
Sodium Chloride / pharmacology
Sodium Hydroxide / pharmacology
Chemical
Reg. No./Substance:
0/Formamides; 1310-73-2/Sodium Hydroxide; 7647-01-0/Hydrochloric Acid; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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