| Changes in messenger RNA abundance of amino acid transporters in rat mammary gland during pregnancy, lactation, and weaning. | |
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MedLine Citation:
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PMID: 19375580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During lactation, the mammary gland increases the needs for nutrients to fulfill the milk production requirements. Among these nutrients, amino acids play an important role for the synthesis of milk proteins. Amino acids are supplied to the mammary gland through amino acid transporters, although some are synthesized in situ. The purpose of this study was to establish the pattern of changes in messenger RNA abundance of the amino acid transporters ASC, system L, EAAC1, GLAST, CAT-1, and Tau in the mammary gland of the rat during different stages of pregnancy and lactation. Rats were fed during pregnancy and lactation a 20% casein diet. Food intake increased significantly during the lactation period. Amino acid transporter ASC expression increased during the first days of pregnancy about 2-fold, and it was increased in a lesser extent again during the peak of lactation. The expression of system L (LAT-1) and CAT-1 transporters was increased only during the lactation period. On the other hand, the expression of the transporters for anionic amino acids EAAC1 and GLAST was low during both stages. Finally, taurine transporter expression decreased during pregnancy; and it was significantly lower during lactation. These results showed that amino acid transporters were not expressed similarly in the mammary gland during pregnancy and lactation, indicating that the expression of these transporters did not respond only to the metabolic needs of the gland but depended on the dietary protein supply and possibly the specific hormonal changes that occur during pregnancy and lactation. |
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Authors:
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Gabriela Alemán; Adriana López; Guillermo Ordaz; Nimbe Torres; Armando R Tovar |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 58 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
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Languages: eng Pagination: 594-601 Citation Subset: IM |
Affiliation:
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Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, DF 14000, México. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Transport Systems
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genetics,
metabolism* Amino Acids / metabolism* Animals Body Weight / physiology Eating / physiology Female Gene Expression Regulation Lactation Mammary Glands, Animal / metabolism* Pregnancy Pregnancy, Animal / genetics, metabolism* RNA, Messenger / genetics, metabolism* Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Amino Acid Transport Systems; 0/Amino Acids; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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