Document Detail


Changes in luteal cells distribution, apoptotic rate, lipid peroxidation levels and antioxidant enzyme activities in buffalo (Bubalus bubalis) corpus luteum.
MedLine Citation:
PMID:  20378285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Buffalo (Bubalus bubalis) is known for its weak/silent estrous behaviour, lower conception rate and longer inter-calving interval as compared to cattle. Understanding the kinetics and functional properties of luteal cells may be helpful to improve reproductive efficiency in the buffalo. Hence the present study was designed to assess the size and distribution of steroidogenic luteal cells along with biochemical properties during different phases of corpus luteum (CL) in the buffalo. The ovaries collected from the local abattoir were classified into three phases, early, mid and late, based on the morphological appearance of the CL as well as the follicles in the ovary. The proportion (%) of the luteal cells (>10microm diameter) increased (P<0.01) from early (30.7+/-1.3) to mid (36.30+/-1.6), and then decreased (P<0.01) in late luteal (31.46+/-1.8) phases. Percentage of small luteal cells (10-20microm diameter) was higher (P<0.05) in early (58.47+/-0.61) and mid (61.29+/-0.67) than late luteal (37.18+/-1.50) phases of CL. However, the percentage of large luteal cells (20-50microm diameter) was higher (P<0.05) only in late (62.82+/-1.50) than early (41.53+/-0.61) and mid (38.71+/-0.67) phases of CL. The average size (microm) of the large luteal cells increased (P<0.05) from early (25.46+/-0.62) to mid (27.15+/-0.5) and late (28.86+/-0.47) luteal phases. The percentage of luteal cells expressing in situ DNA fragmentation was significantly (P<0.05) higher in the late luteal (41.17+/-5.8) than mid-luteal (21.15+/-4.9) phase of the CL. In the early stage, half of the steroidogenic luteal cells had significantly (P<0.05) less 3beta-HSD activity than the other two phases. In the mid stage, the steroidogenic luteal cells had significantly higher (P<0.05) intense 3beta-HSD activity than the other two phases. Further in the late phase, a significant (P<0.05) reduction in intense 3beta-HSD activity was observed in the large luteal cells. The lipid peroxidation (micromol/g of CL) levels were significantly (P<0.05) higher in late luteal (3.46+/-0.2) than the mid-luteal (1.43+/-0.16) phases. The superoxide dismutase and catalase enzyme levels (U/mg of protein) were also significantly (P<0.05) higher in late luteal (0.9+/-0.015 and 3.37+/-0.45, respectively) than the mid-luteal (0.1+/-0.01 and 2.34+/-0.3, respectively) phases. In contrast, the GPx activity (U/mg of protein) decreased significantly (P<0.05) from mid-luteal (1.85+/-0.4) to late luteal (1.22+/-0.2) phases. The present study suggests that (i) the decrease in progesterone levels in late CL may be associated with loss of 3beta-HSD activity in large luteal cells and (ii) demise of the buffalo CL may be mediated by apoptosis despite the high levels of luteal antioxidant enzymes.
Authors:
S Selvaraju; B S Raghavendra; T Siva Subramani; R Priyadharsini; I J Reddy; J P Ravindra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-25
Journal Detail:
Title:  Animal reproduction science     Volume:  120     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-18     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Reproductive Physiology Laboratory, Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India. selvarajuars@rediffmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / analysis,  metabolism*
Apoptosis* / physiology
Buffaloes* / metabolism,  physiology
Catalase / metabolism
Cattle
Cells, Cultured
Corpus Luteum / cytology,  metabolism*,  physiology
Enzymes / metabolism
Female
Glutathione Peroxidase / metabolism
Lipid Peroxidation / physiology*
Luteal Cells / cytology,  metabolism*,  physiology
Superoxide Dismutase / metabolism
Tissue Distribution
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Enzymes; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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