Document Detail


Changes in liver gene expression of Azin1 knock-out mice.
MedLine Citation:
PMID:  20737923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ornithine decarboxylase antizyme inhibitor (AZI) was discovered as a protein that binds to the regulatory protein antizyme and inhibits the ability of antizyme to interact with the enzyme ornithine decarboxylase (ODC). Several studies showed that the AZI protein is important for cell growth in vitro. However, the function of this gene in vivo remained unclear. In our study, we analyzed the transcriptional profiles of livers on the 19th day of pregnancy of Azin1 knock-out mice and wild-type mice using the Agilent oligonucleotide array. Compared to the wild-type mice, in the liver of Azin1 knock-out mice 1812 upregulated genes (fold change > or = 2) and 1466 downregulated genes (fold change < or = 0.5) were showed in the microarray data. Altered genes were then assigned to functional categories and mapped to signaling pathways. These genes have functions such as regulation of the metabolism, transcription and translation, polyamine biosynthesis, embryonic morphogenesis, regulation of cell cycle and proliferation signal transduction cascades, immune response and apoptosis. Real-time PCR was used to confirm the differential expression of some selected genes. Overall, our study provides novel understanding of the biological functions of AZI in vivo.
Authors:
Tao Wan; Yuan Hu; Wenlu Zhang; Ailong Huang; Ken-ichi Yamamura; Hua Tang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  65     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-08-26     Completed Date:  2010-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  519-27     Citation Subset:  IM    
Affiliation:
Key Laboratory of Molecular Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA Primers
DNA, Complementary / genetics
Enzyme Inhibitors / pharmacology
Gene Expression Profiling / methods*
Gene Expression Regulation*
Liver / physiology*
Mice
Mice, Knockout
Oligonucleotide Array Sequence Analysis
Ornithine Decarboxylase / antagonists & inhibitors
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/Enzyme Inhibitors; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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