| Changes in light-induced fluorescence of cervical collagen in guinea pigs during gestation and after sodium nitroprusside treatment. | |
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MedLine Citation:
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PMID: 11776685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Light-induced fluorescence (LIF) of collagen was used to investigate in vivo changes in cervical collagen in guinea pigs during gestation and following sodium nitroprusside treatment. Natural fluorescence of collagen is due to collagen cross-linking molecules that connect single collagen fibers and therefore provide rigidity of the cervical stroma. LIF of cervical collagen was measured from the surface of the exocervix in anesthetized nonpregnant and timed pregnant guinea pigs at different times of gestation with an instrument designed in our lab (Collascope). Measurements were also performed in guinea pigs at midgestation before and 8 hours after intracervical treatment with sodium nitroprusside. Collagen fluorescence decreased significantly as pregnancy progressed, reached lowest values at delivery, and increased gradually postpartum. Treatment with sodium nitroprusside, but not with the vehicle, caused a significant decrease in LIF (p = 0.007). We conclude, that LIF changes in the cervix reflect the gradual cervical softening (ripening) during pregnancy and the return to the rigid state of the cervix postpartum. Cervical softening during pregnancy, and after sodium nitroprusside treatment, is associated with a decrease in collagen cross-links. Measurements of LIF can be used to investigate cervical softening in vivo. |
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Authors:
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C T Fittkow; S Q Shi; E Bytautiene; G Olson; G R Saade; R E Garfield |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of perinatal medicine Volume: 29 ISSN: 0300-5577 ISO Abbreviation: J Perinat Med Publication Date: 2001 |
Date Detail:
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Created Date: 2002-01-04 Completed Date: 2002-05-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0361031 Medline TA: J Perinat Med Country: Germany |
Other Details:
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Languages: eng Pagination: 535-43 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Texas Medical Branch at Galveston, Texas, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cervix Uteri / chemistry* Collagen / analysis, chemistry* Female Fluorescence* Gestational Age Guinea Pigs Light* Nitric Oxide Donors / pharmacology* Nitroprusside / pharmacology* Pregnancy Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Nitric Oxide Donors; 15078-28-1/Nitroprusside; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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