| Changes in intracellular sodium and pH during ischaemia-reperfusion are attenuated by trimetazidine. Comparison between low- and zero-flow ischaemia. | |
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MedLine Citation:
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PMID: 10974217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to investigate whether trimetazidine (TMZ; 10(-6)M), which has been shown to inhibit fatty acid oxidation, reduces the ionic imbalance induced by ischaemia and reperfusion, especially through an attenuation in intracellular changes in H(+) and Na(+). METHODS: Isovolumic rat hearts receiving 5.5 mM glucose and 1.2 mM palmitate as metabolic substrates were exposed to zero-flow ischaemia (TI) or low-flow ischaemia (LFI - coronary flow decreased by an average of 90%) (30 min at 37 degrees C) and then reperfused. 23Na nuclear magnetic resonance (NMR) spectroscopy was used to monitor intracellular Na(+) (Na(+)(i)) and 31P NMR spectroscopy was used to monitor intracellular pH (pH(i)). RESULTS: During LFI the major effect of TMZ was a significant reduction in intracellular acidosis, whereas during TI the main effect of TMZ was a significant reduction in Na(+)(i) gain. In addition, the further gain in Na(+)(i) that occurred during the first minutes of reperfusion following TI, and to a far lesser extent following LFI, was suppressed in TMZ-treated hearts and also suppressed when hearts were perfused without fatty acid. In both LFI and TI, TMZ-induced attenuation of ionic imbalance was associated with a significantly improved recovery of ventricular function on reperfusion, as assessed by a lower increase in diastolic pressure and an increased recovery of developed pressure. CONCLUSION: Our data provide evidence that specific myocardial metabolic modulation plays a significant role in reducing ionic imbalance during ischaemia and reperfusion. |
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Authors:
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H El Banani; M Bernard; D Baetz; E Cabanes; P Cozzone; A Lucien; D Feuvray |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cardiovascular research Volume: 47 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-24 Completed Date: 2000-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 688-96 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiologie Cellulaire, Université Paris XI, Orsay, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Coronary Circulation Fatty Acids / metabolism Hydrogen-Ion Concentration Intracellular Fluid / metabolism Magnetic Resonance Spectroscopy Male Myocardial Ischemia / drug therapy, metabolism Myocardial Reperfusion Injury / metabolism, prevention & control* Oxidation-Reduction Rats Rats, Wistar Sodium / metabolism Trimetazidine / therapeutic use* Vasodilator Agents / therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Vasodilator Agents; 5011-34-7/Trimetazidine; 7440-23-5/Sodium |
| Comments/Corrections | |
Comment In:
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Cardiovasc Res. 2000 Sep;47(4):637-9
[PMID:
10974211
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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