| Changes in intracellular electrolyte concentrations during apoptosis induced by UV irradiation of human myeloblastic cells. | |
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MedLine Citation:
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PMID: 16162654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Decreases in the intracellular concentrations of both K(+) and Cl(-) have been implicated in playing a major role in the progression of apoptosis, but little is known about the temporal relationship between decreases in electrolyte concentration and the key events in apoptosis, and there is no information about how such decreases affect different intracellular compartments. Electron probe X-ray microanalysis was used to determine changes in element concentrations (Na, P, Cl, and K) in nucleus, cytoplasm, and mitochondria in U937 cells undergoing UV-induced apoptosis. In all compartments, the initial stages of apoptosis were characterized by decreases in [K] and [Cl]. The largest decreases in these elements were in the mitochondria and occurred before the release of cytochrome c. Initial decreases in [K] and [Cl] also preceded apoptotic changes in the nucleus. In the later stages of apoptosis, the [K] continued to decrease, whereas that of Cl began to increase toward control levels and was accompanied by an increase in [Na]. In the nucleus, these increases coincided with poly(ADP-ribose) polymerase cleavage, chromatin condensation, and DNA laddering. The cytoplasm was the compartment least affected and the pattern of change of Cl was similar to those in other compartments, but the decrease in [K] was not significant until after active caspase-3 was detected. Our results support the concept that normotonic cell shrinkage occurs early in apoptosis, and demonstrate that changes in the intracellular concentrations of K and Cl precede apoptotic changes in the cell compartments studied. |
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Authors:
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F Arrebola; E Fernández-Segura; A Campos; P V Crespo; J N Skepper; A Warley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-14 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 290 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-11 Completed Date: 2006-03-10 Revised Date: 2007-08-13 |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C638-49 Citation Subset: IM |
Affiliation:
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Electron Microscopy Unit, King's College London, Department of Ophthalmology, The Rayne Institute, St. Thomas' Hospital, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / radiation effects* Cell Line, Tumor Cell Nucleus / chemistry*, radiation effects Chlorine / metabolism Cytoplasm / chemistry* Electrolytes* Electron Probe Microanalysis Humans Lymphoma Mitochondria / chemistry*, radiation effects Phosphorus / metabolism Potassium / metabolism Sodium / metabolism Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Electrolytes; 7440-09-7/Potassium; 7440-23-5/Sodium; 7723-14-0/Phosphorus; 7782-50-5/Chlorine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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