Document Detail


Changes in intestinal microbiota and humoral immune response following probiotic administration in brown trout (Salmo trutta).
MedLine Citation:
PMID:  17313714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the effect of several lactic acid bacteria (LAB) on the humoral response of brown trout (Salmo trutta). LAB groups (Lactococcus (Lc.) lactis ssp. lactis, Lactobacillus (Lb.) sakei and Leuconostoc (Leu.) mesenteroides) were administered orally at 10(6) colony-forming units/g feed to brown trout for 2 weeks, after which fish were switched to an unsupplemented feed. Blood and intestinal samples were taken from the onset of feeding supplemented diets at 1, 2, 3 and 4 weeks. During the LAB-feeding period, Lc. lactis ssp. lactis, Lb. sakei and Leu. mesenteroides persisted in the fish intestines, but the number of LAB slowly decreased in the intestines after changing to the unsupplemented diet. Only Lb. lactis ssp. lactis and Leu. mesenteroides were detected at levels above 1 x 10(2) colony-forming units/g at the end of the fourth week. In comparison to untreated control fish, the alternative complement activity in the serum was found to be significantly greater in all LAB groups at the end of the second week. Groups supplemented with Lc. lactis ssp. lactis and Leu. mesenteroides exhibited an elevated level of lysozyme activity at the end of the third week, but the group supplemented with Lb. sakei did not exhibit any significant change in lysozyme activity. Serum immunoglobulin levels were higher compared with the control group, but there was no significant difference between the LAB and control groups.
Authors:
José Luis Balcázar; Ignacio de Blas; Imanol Ruiz-Zarzuela; Daniel Vendrell; Ana Cristina Calvo; Isabel Márquez; Olivia Gironés; José Luis Muzquiz
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of nutrition     Volume:  97     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-22     Completed Date:  2007-04-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  522-7     Citation Subset:  IM    
Affiliation:
Laboratory of Fish Pathology, University of Zaragoza, Zaragoza, Spain. balcazar@unizar.es
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Aquaculture / methods
Bacteria / growth & development*
Colony Count, Microbial
Complement Pathway, Alternative
Immunoglobulins / biosynthesis,  blood*
Intestines / microbiology*
Lactobacillus / growth & development
Lactococcus lactis / growth & development
Leuconostoc / growth & development
Muramidase / blood
Probiotics*
Trout / immunology*,  microbiology*
Chemical
Reg. No./Substance:
0/Immunoglobulins; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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