Document Detail


Changes in high-energy phosphate metabolism and cell morphology in four models of acute experimental pancreatitis.
MedLine Citation:
PMID:  2009016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies using the isolated ex vivo perfused canine pancreatitis preparation showed that during a 4-hour perfusion pancreatitis (edema, weight gain, hyperamylasemia) can be induced by four different stimuli. The stimuli include the intra-arterial infusion of oleic acid (FFA), a 2-hour period of ischemia before perfusion (ISCH), partial obstruction of the pancreatic duct with secretin stimulation (POSS), and the intra-arterial infusion of cerulein at supramaximal doses (CER). In the present study, changes in high-energy phosphate metabolism, as determined by nuclear magnetic resonance spectroscopy, and changes in cellular structure, determined by light and electron microscopy, were documented for all four models of acute pancreatitis. The control preparations remained stable for the 4-hour perfusion period, with no decrease in adenosine triphosphate (ATP) levels. In the FFA preparations, ATP decreased to 36% of baseline levels during the 4-hour perfusion (p less than 0.001). In the ISCH preparations, ATP decreased to undetectable levels during the 2-hour period of ischemia, but recovered rapidly and remained at baseline levels during the perfusion. ATP levels remained stable in the remaining two models of pancreatitis (POSS, CER). Microscopy demonstrated that the initial injury was located chiefly in the capillaries (swollen endothelium, intravascular thrombi) in the FFA and ISCH preparations. In the POSS and CER preparations, capillary changes were minimal and the injury was located chiefly in the acinar cells (swollen endoplasmic reticulum, zymogen granule depletion, vacuolization). The POSS preparations also showed striking dilation of centroacinar lumens reflecting duct obstruction. In additional studies it was shown that the ATP decline in the FFA preparations could be significantly reduced by pretreatment with free radical scavengers. The morphologic changes could be reduced by free radical scavengers in the FFA and ISCH preparations. Any amelioration of morphologic injury in the POSS preparations was obscured by dilatation of centroacinar lumens in both treated and untreated groups. The morphologic changes in the CER preparations were reduced by treatment with a cholecystokinin inhibitor.
Authors:
I H Nordback; J A Clemens; V P Chacko; J L Olson; J L Cameron
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of surgery     Volume:  213     ISSN:  0003-4932     ISO Abbreviation:  Ann. Surg.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-02     Completed Date:  1991-05-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0372354     Medline TA:  Ann Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland 21209.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adenosine Triphosphate / metabolism*
Animals
Caerulein
Dogs
Free Radicals
Magnetic Resonance Spectroscopy
Models, Biological
Oleic Acid
Oleic Acids
Pancreas / metabolism*,  pathology,  ultrastructure
Pancreatitis / etiology,  metabolism*,  pathology
Perfusion
Secretin
Grant Support
ID/Acronym/Agency:
R01 DK 32435/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Oleic Acids; 112-80-1/Oleic Acid; 1393-25-5/Secretin; 17650-98-5/Caerulein; 56-65-5/Adenosine Triphosphate
Comments/Corrections

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