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Changes in heterogeneous nuclear RNP core polypeptide complements during the cell cycle.
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MedLine Citation:
PMID:  3316247     Owner:  NLM     Status:  MEDLINE    
Mammalian heterogeneous nuclear RNP (hnRNP) subcomplexes are shown to be comprised of 14-17 basic A and B core group polypeptides (chrp) when subjected to two-dimensional immunoblot analysis. These proteins are normally confined to the nucleus but are distributed throughout the cell during mitosis. However, not all of the 17 protein spots are observed for all stages of the cell cycle. HeLa cell populations have been synchronized and the basic hnRNP core protein complement examined during S, G2, mitosis, and G1. During cell division several distinct chrp polypeptide species at 35 and 37 kD appear, while another of 37 kD and a chrp of 38 kD are diminished. These altered chrp complements are not due to any effects induced by thymidine treatment but appear to be physiological changes in the chrp polypeptide modification state. The new charge isomers found during mitosis are not the result of selective phosphorylation of the chrp polypeptides. However the nature of the modifications has yet to be determined. The mitosis-specific modified forms of the chrp polypeptides are found in the cytoplasmic fraction derived from mitotic cell populations. When this fraction is centrifuged upon sucrose density gradients the modified chrp polypeptides sediment from 30-200S in a distribution similar to that of hnRNP complexes isolated from the nuclei of randomly dividing cell populations. RNase digestion experiments indicate that the general substructure of the RNA/protein complexes in mitotic cell cytoplasm is similar to that of nuclear hnRNP isolated from unsynchronized cells or tissue.
G P Leser; T E Martin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  105     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-01-07     Completed Date:  1988-01-07     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2083-94     Citation Subset:  IM    
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
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MeSH Terms
Antibodies, Monoclonal
Cell Cycle*
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Hela Cells / cytology
Heterogeneous-Nuclear Ribonucleoproteins
Molecular Weight
Ribonucleoproteins / metabolism*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Heterogeneous-Nuclear Ribonucleoproteins; 0/Ribonucleoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1987
Volume: 105 Issue: 5
First Page: 2083 Last Page: 2094
ID: 2114836
Publisher Id: 88059241
PubMed Id: 3316247

Changes in heterogeneous nuclear RNP core polypeptide complements during the cell cycle

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