Document Detail


Changes in functional activities of plasma fibrinogen after treatment with methylene blue and red light.
MedLine Citation:
PMID:  12702176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Methylene blue (MB) plus light treatment used for virus inactivation of human plasma units may lead to changes in the functional activities of fibrinogen. STUDY DESIGN AND METHODS: Single-donor units of fresh plasma were treated with 1.0 microM MB and a red light dose of 48 J per cm2. The effects of MB plus red light treatment on fibrinogen clottability, fibrin polymerization and gelation, clot stabilization, and fibrinolysis were studied. RESULTS: The concentration of clottable fibrinogen was unchanged during MB plus red light treatment, but a light-dose-dependent decrease of the concentration of functional fibrinogen was found. The initial release rate of fibrinopeptide A was slightly increased after MB plus red light treatment. Turbidity measurements of fibrin gel showed prolonged clotting time, lower fibrin fiber mass-to-length ratio, and slightly smaller fiber diameter. At a given clotting time, a gel with lower fibrin fiber mass-to-length ratio was produced. Clot stability and fibrinolysis remained normal. l-Histidine added to plasma before MB plus red light treatment normalized the thrombin-induced coagulation time in a dose-dependent way. CONCLUSION: MB plus red light treatment affected the polymerization and gelation phase of fibrin. A tighter fibrin gel structure was formed. No effect on stabilization of fibrin clot or fibrinolysis was found.
Authors:
Anna-Maija Suontaka; Margareta Blombäck; John Chapman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion     Volume:  43     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-18     Completed Date:  2003-05-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  568-75     Citation Subset:  IM    
Affiliation:
Department of Surgical Sciences/Blood Coagulation Research, Clinical Chemistry Building, Karolinska Hospital, SE-171 76 Stockholm, Sweden. Anna-Maija.Suontaka@ks.se
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MeSH Terms
Descriptor/Qualifier:
Batroxobin / pharmacology
Blood / drug effects
Blood Coagulation
Factor XIII / metabolism
Fibrin / analysis
Fibrinogen / analysis,  drug effects*,  physiology,  radiation effects*
Fibrinolysis
Fibrinopeptide A / analysis
Gels
Humans
Light*
Methylene Blue / therapeutic use*
Nephelometry and Turbidimetry
Osmolar Concentration
Thrombin / pharmacology
Virus Inactivation*
Whole Blood Coagulation Time
Chemical
Reg. No./Substance:
0/Gels; 25422-31-5/Fibrinopeptide A; 61-73-4/Methylene Blue; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen; 9013-56-3/Factor XIII; EC 3.4.21.-/Batroxobin; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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