Document Detail

Changes in free amino acid and sugar levels of dried figs during aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus.
MedLine Citation:
PMID:  18816056     Owner:  NLM     Status:  MEDLINE    
An aqueous slurry of gamma-irradiated sterilized dried figs was inoculated with toxigenic strains of Aspergillus flavus and Aspergillus parasiticus. During incubation at 28 degrees C, pH, fructose, glucose, and free amino acids were determined by high-performance liquid chromatography and liquid chromatography (LC)/mass spectrometry, respectively, over 13 time points (1-20 days). At the same 13 time points using a LC/time-of-flight mass spectrometry screening method, aflatoxin B 1 and other secondary metabolites were simultaneously monitored. During the course of incubation, the pH significantly decreased and aflatoxin B 1 formation correlated with a reduction in proline content for both fungi. Of the 22 free amino acids that were monitored, only proline and cystine were found to be critical in supporting aflatoxin production. Levels of fructose and glucose steadily declined during incubation, until glucose was almost exhausted after 21 days. These time-course experiments confirmed the need for carbon and nitrogen sources for aflatoxin production in dried figs, and the favorable composition of figs as a fungal growth medium.
Hamide Z Senyuva; John Gilbert; Sebnem Oztürkoglu; Süreyya Ozcan; Neslihan Gürel
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Publication Detail:
Type:  Journal Article     Date:  2008-09-25
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-15     Completed Date:  2008-12-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9661-6     Citation Subset:  IM    
Ankara Test and Analysis Laboratory, Scientific and Technological Research Council of Turkey, Konya Yolu No. 67, Besevler/Ankara 06330, Turkey.
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MeSH Terms
Aflatoxin B1 / metabolism*
Amino Acids / metabolism*
Aspergillus / chemistry,  metabolism*
Chromatography, High Pressure Liquid
Ficus / chemistry,  metabolism*
Fructose / metabolism*
Glucose / metabolism*
Reg. No./Substance:
0/Amino Acids; 1162-65-8/Aflatoxin B1; 30237-26-4/Fructose; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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