| Changes in fluoride sensitivity during in vitro senescence of normal human oral cells. | |
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MedLine Citation:
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PMID: 16161253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously reported that sodium fluoride (NaF) showed slightly higher cytotoxicity against human oral tumor cell lines than normal human oral cells. Possible changes in the NaF sensitivity of three normal human oral cell types (gingival fibroblast HGF, pulp cell HPC, periodontal ligament fibroblast HPLF) during in vitro ageing were investigated in the present study. When these cells were subcultured at 1:4 split ratio every week, their saturation density declined with increasing population doubling level (PDL), and they ceased to divide when they reached 20 PDL. Mitochondrial function, evaluated by MTT stainability per cell basis, was elevated at the terminal phase. NaF dose-dependently reduced the viable cell number, but did not show any beneficial (growth promoting) effect (so-called "hormesis") at lower concentrations. NaF produced large DNA fragments, without induction of internucleosomal DNA fragmentation, possibly due to weak activation of caspases -3, -8 and -9. Higher concentrations of NaF were required to reduce the number of viable senescent cells than younger cells, indicating that cells become resistant to cytotoxicity of NaF with in vitro ageing. |
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Authors:
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Rie Satoh; Kaori Kishino; Sufi Reza Md Morshed; Fumitoshi Takayama; Sumiko Otsuki; Fumika Suzuki; Ken Hashimoto; Hirotaka Kikuchi; Hirofumi Nishikawa; Toshikazu Yasui; Hiroshi Sakagami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 25 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2005 May-Jun |
Date Detail:
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Created Date: 2005-09-14 Completed Date: 2005-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 2085-90 Citation Subset: IM |
Affiliation:
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Department of Dental Pharmacology, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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drug effects Cell Growth Processes / drug effects Cells, Cultured Child DNA Fragmentation / drug effects Dental Pulp Cavity / cytology, drug effects Female Fibroblasts / cytology, drug effects Gingiva / cytology, drug effects Humans Mouth / cytology*, drug effects* Nucleosomes / drug effects Periodontal Ligament / cytology, drug effects Sodium Fluoride / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Nucleosomes; 7681-49-4/Sodium Fluoride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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