Document Detail


Changes in fatty acid composition during development of tissues of coconut (Cocos nucifera L.) embryos in the intact nut and in vitro.
MedLine Citation:
PMID:  11432910     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intact coconuts were germinated in situ and compared with excised zygotic embryos germinated in vitro. The growth of the embryonic tissue and their fatty acid compositions were measured. Haustoria, plumules and radicles of coconuts germinated in situ grew continuously and proportionately throughout the 120 d experiment with haustauria increasing to 45 g x nut(-1) and weighing 4-5-fold more than the other two tissues. The plumules and radicles of the seedlings cultured in vitro also grew continuously but the haustoria grew sporadically between 15 d and 75 d in culture and, at 250 mg x nut(-1) after 75 d, were smaller than the other two tissues. All the tissues of the nuts grown in situ contained significant amounts of lauric acid, the acid characteristic of coconut oil, as well as longer chain saturated and unsaturated fatty acids. The content of medium and long chain fatty acids increased in all growing tissues as the experiment proceeded, especially the haustorium which contained 24-35% of its fatty acid as lauric acid; the fat content of solid endosperm reduced during this period. Seedlings grown in vitro, on the other hand, failed to accumulate lauric acid in any of their tissues (haustorium contained 6-11% of its fatty acid as lauric acid). The results may have implications for the design of growth media for growing zygotic and somatic cultures of coconut and may provide a marker for successful germination.
Authors:
A López-Villalobos; P F Dodds; R Hornung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental botany     Volume:  52     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-02     Completed Date:  2001-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  933-42     Citation Subset:  IM    
Affiliation:
The T.H. Huxley School, Imperial College at Wye, University of London, Wye, Ashford, Kent TN25 5AH, UK.
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MeSH Terms
Descriptor/Qualifier:
Cocos / chemistry*,  growth & development
Fatty Acids / chemistry*
Germination
Seeds
Chemical
Reg. No./Substance:
0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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