| Changes in extracellular pH affect glycine receptor channels expressed in HEK 293 cells. | |
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MedLine Citation:
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PMID: 20359478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycine receptors are expressed throughout the central nervous system working for inhibitory neurotransmission. Since fluctuations of the blood pH value occur under certain physiological and pathological conditions, we investigated the influence of the extracellular pH on glycine homomeric and heteromeric receptor functions using patch clamp in combination with the fast agonist application technique. Our results demonstrated that both alpha1 homomeric and alpha 1 beta heteromeric glycine receptors were remarkably inhibited under acidic extracellular pH. Under alkaline extracellular pH 8.5, there was also a negative functional effect. Desensitization was accelerated depending on pH and a rebound current was observed at an extremely acidic pH value. In double-pulse experiments on alpha1 homomeric receptors, a more rapid recovery of the glycinergic current was shown at pH 4.5 compared to current induced at a physiological pH of 7.2. Our study provided a potential cause for the impaired function of the glycine receptor channels during pH fluctuations occurring in the central nervous system, especially under pathological conditions such as epileptic seizure or ischaemia. |
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Authors:
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Yun-Ping Song; Friedrich Schlesinger; Daniela Ragancokova; Roger Calixto; Reinhard Dengler; Klaus Krampfl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-30 |
Journal Detail:
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Title: European journal of pharmacology Volume: 636 ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 59-64 Citation Subset: IM |
Copyright Information:
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(c) 2010. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Neurology and Clinical Neurophysiology, Hannover Medical School, Germany. igosong@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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pharmacology Cell Line Cell Membrane / metabolism Electric Conductivity Extracellular Space / metabolism* Gene Expression Regulation* Glycine / metabolism* Humans Hydrogen-Ion Concentration Ion Channels / agonists, chemistry, metabolism* Kinetics Protons Receptors, Glycine / agonists, chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/GLRB protein, human; 0/Ion Channels; 0/Protons; 0/Receptors, Glycine; 0/glycine receptor alpha1; 56-40-6/Glycine; 56-41-7/Alanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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