Document Detail

Changes in expression of fibroblast surface antigen (SFA) during cytodifferentiation and heterokaryon formation.
MedLine Citation:
PMID:  964519     Owner:  NLM     Status:  MEDLINE    
Fibroblast surface antigen (SF antigen, SFA) is a major glycoprotein antigen detected in connective tissue cells (primitive mesenchymal cells, fibroblasts, and astroglial cells). In this study the expression of SFA was followed during differentiation of the mesenchymal cells of the mouse metanephros and during heterokaryon formation produced by Sendai-virus induced fusion of human fibroblasts and chick red blood cells. It was demonstrated by immunofluorescence that SFA was lost from the kidney mesenchymal cells when they differentiate into epithelial cells of the secretory tubuli. During this process SFA became detectable in the basement membrane formed around the tubuli. In cell fusion experiments human SFA which was present as fibrillar network on the surface of cultured fibroblasts, was gradually lost from the heterokaryons when the incorporated chick nuclei became activated. These two sets of experiments indicate that SFA can be used as a phenotypic marker of cytodifferentiation.
J Wartiovaara; S Stenman; A Vaheri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  5     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1976-11-21     Completed Date:  1976-11-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  85-9     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation*
Cell Membrane / immunology*
Chick Embryo
Culture Techniques
Embryo, Mammalian / immunology
Erythrocytes / immunology
Fibroblasts / immunology*
Glycoproteins / immunology
Hybrid Cells / immunology*
Kidney / embryology
Reg. No./Substance:
0/Antigens; 0/Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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