| Changes in elemental concentrations are associated with early stages of apoptosis in human monocyte-macrophages exposed to oxidized low-density lipoprotein: an X-ray microanalytical study. | |
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MedLine Citation:
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PMID: 10398148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examines ion homeostasis in monocyte-macrophages committed to death by apoptosis. X-ray microanalysis has been used to demonstrate that intracellular concentrations of potassium decreased whilst those of sodium increased following 3 h of exposure to 100 microg/ml of oxidized low-density lipoprotein (LDL) in vitro. In contrast, the maximal incidence of cell death, as determined by the inability to exclude trypan blue, was not seen until 24 h of exposure. At 12 h, less than 1 per cent of cells were stained using terminal transferase-mediated DNA nick-end labelling, which is generally accepted as a marker of late stages in the apoptotic pathway. This is the first demonstration of early perturbations of ion homeostasis in monocyte-macrophages exposed to concentrations of oxidized LDL known to cause apoptosis. |
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Authors:
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J N Skepper; I Karydis; M R Garnett; L Hegyi; S J Hardwick; A Warley; M J Mitchinson; N R Cary |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pathology Volume: 188 ISSN: 0022-3417 ISO Abbreviation: J. Pathol. Publication Date: 1999 May |
Date Detail:
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Created Date: 2000-02-15 Completed Date: 2000-02-15 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 0204634 Medline TA: J Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 100-6 Citation Subset: IM |
Copyright Information:
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Copyright 1999 John Wiley & Sons, Ltd. |
Affiliation:
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Multi-Imaging Centre, University of Cambridge, Department of Anatomy, Tennis Court Road, Cambridge CB2 3DY, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Chlorides / metabolism Electron Probe Microanalysis Homeostasis Humans In Situ Nick-End Labeling Iron / metabolism* Lipoproteins, LDL / metabolism, pharmacology* Macrophages / drug effects, metabolism*, physiology Magnesium / metabolism Microscopy, Electron, Scanning Transmission Phosphorus / metabolism Potassium / metabolism Sodium / metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Lipoproteins, LDL; 7439-89-6/Iron; 7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-23-5/Sodium; 7723-14-0/Phosphorus |
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