Document Detail

Changes in elemental concentrations are associated with early stages of apoptosis in human monocyte-macrophages exposed to oxidized low-density lipoprotein: an X-ray microanalytical study.
MedLine Citation:
PMID:  10398148     Owner:  NLM     Status:  MEDLINE    
This study examines ion homeostasis in monocyte-macrophages committed to death by apoptosis. X-ray microanalysis has been used to demonstrate that intracellular concentrations of potassium decreased whilst those of sodium increased following 3 h of exposure to 100 microg/ml of oxidized low-density lipoprotein (LDL) in vitro. In contrast, the maximal incidence of cell death, as determined by the inability to exclude trypan blue, was not seen until 24 h of exposure. At 12 h, less than 1 per cent of cells were stained using terminal transferase-mediated DNA nick-end labelling, which is generally accepted as a marker of late stages in the apoptotic pathway. This is the first demonstration of early perturbations of ion homeostasis in monocyte-macrophages exposed to concentrations of oxidized LDL known to cause apoptosis.
J N Skepper; I Karydis; M R Garnett; L Hegyi; S J Hardwick; A Warley; M J Mitchinson; N R Cary
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  188     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  100-6     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Multi-Imaging Centre, University of Cambridge, Department of Anatomy, Tennis Court Road, Cambridge CB2 3DY, U.K.
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MeSH Terms
Chlorides / metabolism
Electron Probe Microanalysis
In Situ Nick-End Labeling
Iron / metabolism*
Lipoproteins, LDL / metabolism,  pharmacology*
Macrophages / drug effects,  metabolism*,  physiology
Magnesium / metabolism
Microscopy, Electron, Scanning Transmission
Phosphorus / metabolism
Potassium / metabolism
Sodium / metabolism
Time Factors
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Chlorides; 0/Lipoproteins, LDL; 7439-89-6/Iron; 7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-23-5/Sodium; 7723-14-0/Phosphorus

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