Document Detail


Changes in the electrophoretic profiles of gill mucus proteases of the eastern oyster Crassostrea virginica in response to infection by the turbellarian Urastoma cyprinae.
MedLine Citation:
PMID:  10772329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Urastoma cyprinae occurs on the gills of various bivalves species, including the eastern oyster Crassostrea virginica. While the worm is known to cause severe gill disruption in mussels, no evidence of this nature has been described for oysters. Nonetheless, high levels of U. cyprinae have been reported in oysters, which may, in turn, reduce the oyster's overall condition. U. cyprinae is strongly attracted to oyster gill mucus, which is suggested to play an active role in the worm's feeding activities. Furthermore, host mucus contains many active components, including proteases, which have been suggested to play a defensive role against invading organisms. It follows, therefore, that some of the interactions between U. cyprinae and oysters take place in host gill mucus. Studies were undertaken to determine whether the presence of U. cyprinae altered the electrophoretic profiles of oyster gill mucus, using proteases as indicators. Findings reveal that oyster gill mucus contains three proteases, a putative acid protease at 96 kDa, a zinc metalloprotease at 64 kDa, and a serine protease at 33 kDa. Results based on experiments using mucus preparations extracted from infected and noninfected oysters, along with those using lyophilized mucus incubated with live U. cyprinae, confirm that the presence of U. cyprinae alters the protease composition of gill mucus. The present data demonstrate that both U. cyprinae and host gill mucus actively secrete proteases. While the precise roles of these enzymes still need to be defined, one of their functions may be associated with digestion-related activities induced by the worm.
Authors:
N T Brun; N W Ross; A D Boghen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  75     ISSN:  0022-2011     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-05-16     Completed Date:  2000-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  163-70     Citation Subset:  IM    
Affiliation:
Département de Biologie, Université de Moncton, New Brunswick, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophoresis, Polyacrylamide Gel
Endopeptidases / chemistry*
Gills / enzymology*
Molecular Weight
Mucus / enzymology
Ostreidae / parasitology*
Turbellaria*
Chemical
Reg. No./Substance:
EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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